Dataset Information

ITRAQ-based proteomic analysis reveals high similarity of protein expression in ALNA with ileum PPs of Bactrian camel (Camelus bactrianus)

ABSTRACT: Bactrian camel (Camelus bactrianus) is a large, even-toed ungulate native to the desert areas of Central Asia. Aggregated lymphoid nodules area (ALNA) is known as the special anatomical structure of a Bactrian camel’s stomach that is found in the cardiac glandular area of the abomasum. To advance the exploration of the mucosal immune function of this anatomical feature within the gastrointestinal tract, we quantified the expressed proteins in ALNA using iTRAQ-based proteomic analysis in 6 Bactrian camels. In the experiment, we sampled the reticular mucosal folds region (RMFR) and longitudinal mucosal folds region (LMFR) separately from the ALNA and compared their proteomic quantification results with the samples from the Peyer’s patches (PPs).

ORGANISM(S): Camelus Bactrianus

SUBMITTER: Wenhui Wang

PROVIDER: PXD041670 | iProX | Thu Apr 20 00:00:00 BST 2023

REPOSITORIES: iProX

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Publications

Similar proteome expression profiles of the aggregated lymphoid nodules area and Peyer's patches in Bactrian camel.

Cheng Yujiao Y Ren Yan Y Wang Wenhui W Zhang Wangdong W

BMC genomics 20231011 1

<h4>Background</h4>The presence of Aggregated Lymphoid Nodules Area (ALNA) is a notable anatomical characteristic observed in the abomasum of Bactrian camels. This area is comprised of two separate regions, namely the Reticular Mucosal Folds Region (RMFR) and the Longitudinal Mucosal Folds Region (LMFR). The histological properties of ALNA exhibit significant similarities to those of Peyer's patches (PPs) found in the gastrointestinal system. The functional characteristics of ALNA were examined ...[more]

PMID: 37821839

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Project description:Stomach and intestinal epithelial cells are maintained by the activity of stem cells located in the isthmus and crypt, respectively1,2. Recent studies have demonstrated a surprisingly conserved role for Wnt signaling in stomach and intestinal development and stem cells3,4. Although accumulating evidence suggests that intestinal stromal cells secrete Wnt ligands to promote stem cell renewal5-10, the source of stomach Wnt ligands is still unclear. Moreover, how these gastrointestinal stem cell niche signals are produced is currently unknown. By performing single cell analysis of gastrointestinal stromal cells, we identified cell populations with transcriptome signatures that are conserved between the stomach and intestine. In close proximity to gastrointestinal epithelial cells, these cells highly expressed pericyte markers and Wnt ligands. They also were enriched for Hh signaling, which plays a key role in gut development11,12. A recent study has shown that intestinal pericryptal cells co-express Hh target and Wnt ligand genes8. To define their relationship, we analyzed mice with Hh gain of function in the pericyte-like stromal cells conserved between the stomach and intestine, and found increased levels of Wnt ligands, supporting Hh regulation of stromal Wnt ligand expression. Moreover, utilizing Sufu and Spop double knockout mice, which stabilized GLI2, a key Hh mediator in the gut, we were able to map GLI2 binding sites genome-wide and analyze super enhancers. This work demonstrates GLI2 activation of stromal Wnt ligands through enhancers that are conserved between the stomach and intestine. To determine the significance of Wnt secreting gastrointestinal stromal cells, we genetically inhibited Wnt secretion from the perictye-like or broad stromal cells, demonstrating their roles in gastrointestinal regeneration and development, respectively. Our work not only identifies the conserved gastrointestinal stromal niche cell populations but also reveals their underlying signaling and epigenetic mechanisms.

Project description:Transcription factor Foxq1 controls mucin gene expression and granule content in mouse stomach surface mucous cells ; Background and Aims: The gastric mucosa provides a stringent epithelial barrier and produces acid and enzymes that initiate digestion. In this regenerating tissue, progenitors differentiate continually into 4 principal specialized cell types, yet underlying mechanisms of differentiation are poorly understood. We identified stomach-restricted expression of the forkhead transcription factor FOXQ1. Methods: We used a combination of genetic, histochemical, ultrastructural and molecular analysis to study gastric cell lineages with respect to FOXQ1. Results: Within the developing and adult gastrointestinal tract, Foxq1 mRNA is restricted to the stomach, expressed prominently in foveolar (pit) cells, the abundant mucin-producing cells that line the mucosal surface, and required for their complete differentiation. Mice carrying Foxq1 coding mutations show virtual absence of mRNA and protein for the backbone of the predominant stomach mucin, MUC5AC. These observations correspond to a paucity of foveolar-cell secretory vesicles and notable loss of stomach but not intestinal mucus. Transcriptional profiling identified a surprisingly restricted set of genes with altered expression in Foxq1 mutant stomachs. MUC5AC is a highly tissue-restricted product that similarly depends on FOXQ1 in its other major site of expression, conjunctival goblet cells. Conclusions: Taken together, these observations imply that promotion of gastric MUC5AC synthesis is a primary, cell-autonomous function of FOXQ1. This study is the first to implicate a transcription factor in terminal differentiation of foveolar cells and begins to define the requirements to assemble highly specialized organelles and cells in the gastric mucosa. DOI: 10.1053/j.gastro.2008.04.019 Experiment Overall Design: RNA from stomach antrum from 2 of each Satin, Beige, and BL6 mice were compared using microarray analysis.

Dataset Information

ITRAQ-based proteomic analysis reveals high similarity of protein expression in ALNA with ileum PPs of Bactrian camel (Camelus bactrianus)

Publications

Similar proteome expression profiles of the aggregated lymphoid nodules area and Peyer's patches in Bactrian camel.

Similar Datasets

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