Dataset Information

Proteome of Apis Laboriosa Honey against Salmonella enterica serovar Typhimurium

ABSTRACT: Proteome of Apis Laboriosa Honey against Salmonella enterica serovar Typhimurium

ORGANISM(S): Salmonella Enterica Subsp. Enterica Serovar Typhimurium Str. Atcc 14028

SUBMITTER: Wenchao Yang

PROVIDER: PXD041706 | iProX | Fri Apr 21 00:00:00 BST 2023

REPOSITORIES: iProX

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Publications

Antioxidant and antibacterial activity of Apis laboriosa honey against Salmonella enterica serovar Typhimurium.

Tan Weihua W Tian Yuanyuan Y Zhang Qingya Q Miao Siwei S Wu Wenrong W Miao Xiaoqing X Kuang Haiou H Yang Wenchao W

Frontiers in nutrition 20230511

Salmonella enterica serovar Typhimurium (S. Typhimurium) is a common food-borne pathogen that commonly causes gastroenteritis in humans and animals. Apis laboriosa honey (ALH) harvested in China has significant antibacterial activity against Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. We hypothesize that ALH has antibacterial activity against S. Typhimurium. The physicochemical parameters, minimum inhibitory and bactericidal co ...[more]

PMID: 37252242

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Project description:InvF ChIP-chip on Salmonella enterica serovar Typhimurium SL1344 using anti-Myc antibody against strain with chromosomally 9Myc-tagged InvF (IP samples) and wildtype strain (mock IP samples) Salmonella enterica serovar Typhimurium causes a range of diseases from self-limiting gastroenteritis to life-threatening systemic infections. Its complex infection process is initiated by the invasion of the intestinal epithelial monolayer by means of a type three secretion system. InvF is one of the key regulators governing the invasion of epithelial cells. By mapping the InvF regulon, i.e. locating its direct target genes, the gene network underlying invasion can be further examined, including identifying possible new effector-encoding genes. In order to map the InvF regulon, we performed chromatin immunoprecipitation combined with tiling microarray analysis (ChIP-chip) and compared expression of the identified target genes in an invF mutant and a wildtype strain. In addition, the promoter regions of these target genes were searched for the presence of an InvF recognition site. Finally, a query-driven biclustering method, combined with a microarray compendium containing publically available S. Typhimurium gene expression data, was applied as an in silico validation technique for functional relatedness between newly identified target genes and known invasion genes. As expected, under invasion inducing conditions, InvF activates the expression of invasion chaperone encoding sicA and the effector-encoding genes sopB, sopE, sopE2 and sopA by binding their promoter region. Newly identified InvF targets are steB, encoding a secreted effector, and STM1239. The presence of an InvF recognition site in the promoter regions of these target genes further supports this observation. In addition, the query-driven biclustering method revealed similarities in expression profiles between STM1239 and known InvF regulated invasion genes over a range of experimental conditions. In conclusion, we here deliver the first evidence for direct binding of InvF to the promoter regions of sopA and sopE2, and associate genes encoding a secreted effector (steB) and a putative novel effector (STM1239) with the Salmonella invasion regulator InvF. Three IP samples (from three biological replicates using anti-Myc antibody against Salmonella Typhimurium SL1344 strain encoding chromosomally 9Myc-tagged InvF) and three control mock IP samples (from three biological replicates using anti-Myc antibody against Salmonella Typhimurium SL1344 wildtype strain) were labeled with Cy5 and hybridized against a common genomic DNA reference, labeled with Cy3, on 6 S. Typhimurium LT2 whole genome tiling arrays

Dataset Information

Proteome of Apis Laboriosa Honey against Salmonella enterica serovar Typhimurium

Publications

Antioxidant and antibacterial activity of <i>Apis laboriosa</i> honey against <i>Salmonella enterica</i> serovar Typhimurium.

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