Quantitative proteomics analysis based on data-independent acquisition reveals the effect of Shenling Baizhu powder (SLP) on protein expression in MAFLD rat liver tissue

ABSTRACT: Metabolic fatty liver disease (MAFLD) has become the most common chronic liver disease worldwide, with steatosis as the underlying pathology. At the same time, MAFLD is also a high-risk factor for the development of metabolic diseases. Shenling Baizhu powder (SLP) is a traditional Chinese herbal formula with good clinical efficacy in the treatment of MAFLD. However, its molecular mechanism for the treatment of MAFLD is still not fully understood. This study used quantitative proteomics analysis to reveal the mechanism of action of SLP in the treatment of MAFLD by discovering the effect of SLP on protein expression in the liver tissue of MAFLD rats. Orbitrap high-resolution liquid chromatography mass spectrometry (Q-Orbitrap LC-MS/MS) was used to identify the blood-entering compounds of SLP. The 18 SD male rats were randomly divided into 3 groups (n=6): Control group, HFD group and SLP group. The HFD group and SLP group were established as MAFLD rat models by feeding them with a high-fat diet for 4 weeks. Then, the SLP group was treated with SLP (10.89g / kg / d) for 3 weeks. Measurement of biochemical parameters and pathological status of the liver in each group of rats. Rat liver tissue was analyzed using DIA-based quantitative proteomics and validated by western blotting.

ORGANISM(S): Rattus Norvegicus

SUBMITTER: Tao Liu  

PROVIDER: PXD042049 | iProX | Sun May 07 00:00:00 BST 2023

REPOSITORIES: iProX