Project description:ActD timecourse in HeLa cells, HeLa RNF219 KO cells, HeLa RNF219 KO cells overexpressing 3xFLAG-SBP-RNF219-WT or 3xFLAG-SBP-RNF219-mut

| PRJNA721707 | ENA

WT and ATRX KO

Project description:WT and ATRX KO

| PRJNA254835 | ENA

RNA-seq using human SH-SY5Y neuroblastoma cells used for SILAC experiment

Project description:To measure translational efficiency in FMRP depletion, we purified RNAs from either wild-type or FMR1-knockout (FMR1-KO) SH-SY5Y cells generated for SILAC coupled to LC-MS/MS analysis and performed RNA-seq to quantitate mRNA abundance to normalize their protein abundance.

2022-10-30 | GSE197221 | GEO

SCN circadian proteomes of miR-132/212 KO and WT mice by super-SILAC-based quantitative mass spectrometry

Project description:To identify potential mechanisms underlying the phenotype of miR-132/212 KO mice under short photoperiods, we used quantitative mass spectrometry to analyze the SCN proteomes of miR-132/212 KO and WT mice under a 8:16 LD schedule at 4 time points, spaced 6h apart, across a 24h cycle (n=4 per time point per genotype). Quantification was achieved using a spike-in reference of three murine neural cell lines, including Neuro2A, and the adult mouse hypothalamic cell lines mHypoA-2/28 (CLU188) and mHypoA-SCN mix (CLU497) that had been labeled by SILAC (stable isotope labeling by amino acids in cell culture).

2017-04-24 | PXD003635 | Pride

Analysis of HeLa cells after transfection with miR-1, miR-124 and miR-181.

Project description:Analysis of HeLa cells at 24 hours after transfection with wild type miR-1, miR-124, miR-181 versus control transfected HeLa cells. Results were compared to protein down-regulation at 48 hours measured by SILAC-MS. Analysis of HeLa cells at 24 hours after transfection with wild type miR-1, miR-124, miR-181 versus control transfected HeLa cells. Results were compared to protein down-regulation at 48 hours measured by SILAC-MS.

2010-05-25 | E-GEOD-11968 | biostudies-arrayexpress

ADAR1-editing in HeLa, p150-KO and ADAR1-KO transcriptomes

Project description:ADAR1-editing in HeLa, p150-KO and ADAR1-KO transcriptomes

| PRJNA473839 | ENA