Dataset Information

Data of plasma proteome in two groups of healthy people at different ages

ABSTRACT: The plasma proteome data of 10 healthy young people and the proteome data of 10 healthy senior people. The sample processing did not remove the high-abundance protein, and the data was acquired by DDA. The instrument used was Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens Mus Musculus

SUBMITTER: Youhe Gao

PROVIDER: PXD042215 | iProX | Sun May 14 00:00:00 BST 2023

REPOSITORIES: iProX

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Publications

Global chemical modifications comparison of human plasma proteome from two different age groups.

Liu Yongtao Y Pan Xuanzhen X Zhao Mindi M Gao Youhe Y

Scientific reports 20200914 1

In this study, two groups of human plasma proteome at different age groups (old and young) were used to perform a comparison of global chemical modifications, as determined by tandem mass spectrometry (MS/MS) combined with non-limiting modification identification algorithms. The sulfhydryl in the cysteine A total of 4 molecular modifications were found to have significant differences passing random grouping tests: the succinylation and phosphorylation modification of cysteine (Cys, C) and the mo ...[more]

PMID: 32929118

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Project description:Genome wide DNA methylation profiling of isolated monocyte samples from healthy Kenyan children, the same children during an episode of acute malaria, healthy Kenyan adults, and healthy adults from the United States. The Illumina Infinium MethylationEPIC BeadChip microarray was used to obtain DNA methylation profiles across approximately 860,000 CpGs in negatively selected monocyte samples. Samples included monocytes from 8 children from western Kenya obtained while healthy and matching samples from the same 8 Kenyan children obtained during an episode of acute uncomplicated Plasmodium falciparum malaria, 8 healthy malaria-immune adults from western Kenya, and 8 healthy malaria-naive adults from the US. Abstract -- Background: Age-related changes in adaptive and innate immune cells have been associated with a decline in effective immunity and chronic, low-grade inflammation. Epigenetic, transcriptional, and functional changes in monocytes occur with aging, though most studies to date have focused on differences between young adults and the elderly in populations with European ancestry; few data exist regarding changes that occur in circulating monocytes during the first few decades of life or in African populations. We analyzed DNA methylation profiles, cytokine production, and inflammatory gene expression profiles in monocytes from young adults and children from western Kenya. Results: We identified several hypo- and hyper-methylated CpG sites in monocytes from Kenyan young adults vs. children that replicated findings in the current literature of differential DNA methylation in monocytes from elderly persons vs. young adults across diverse populations. Differentially methylated CpG sites were also noted in gene regions important to inflammation and innate immune responses. Monocytes from Kenyan young adults vs. children displayed increased production of IL-8, IL-10, and IL-12p70 in response to TLR4 and TLR2/1 stimulation as well as distinct inflammatory gene expression profiles. Conclusions: These findings complement previous reports of age-related methylation changes in isolated monocytes and provide novel insights into the role of age-associated changes in innate immune functions.

Dataset Information

Data of plasma proteome in two groups of healthy people at different ages

Publications

Global chemical modifications comparison of human plasma proteome from two different age groups.

Similar Datasets

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