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ZASP：A Robust Method for Proteomics Sample Preparation

ABSTRACT: ZASP can effectively remove SDS, SDC, TritonX-100 and other denaturing reagents. • ZASP has short precipitation time, high protein recovery and identification number. • Compared with acetone and SP3 precipitation method, ZASP has remarkable performance. • ZASP is particularly suitable for complex samples, simplifying the sample processing process and increasing the number of protein identifications.

ORGANISM(S): Homo Sapiens Mus Musculus

SUBMITTER: Fuchu He

PROVIDER: PXD042276 | iProX | Tue May 16 00:00:00 BST 2023

REPOSITORIES: iProX

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ZASP: A Highly Compatible and Sensitive ZnCl2 Precipitation-Assisted Sample Preparation Method for Proteomic Analysis.

Shao Xianfeng X Huang Yuanxuan Y Xu Rong R He Qiqing Q Zhang Min M He Fuchu F Wang Dongxue D

Molecular & cellular proteomics : MCP 20240906 10

Universal sample preparation for proteomic analysis that enables unbiased protein manipulation, flexible reagent use, and low protein loss is required to ensure the highest sensitivity of downstream liquid chromatography-mass spectrometry (LC-MS) analysis. To address these needs, we developed a ZnCl2 precipitation-assisted sample preparation method (ZASP) that depletes harsh detergents and impurities in protein solutions prior to trypsin digestion via 10 min of ZnCl2 and me ...[more]

PMID: 39243857

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