Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteome of norfloxacin-resistant Edwardsiella tarda

ABSTRACT: we propagate norfloxacin-resistant strain, LTB4-R, through sequential propagations of norfloxacin-sensitive LTB4 in TSB broth medium with norfloxacin. LTB4-R had 128-fold minimum inhibitory concentrations (MIC) of LTB4-S. To profile the differential protein expression between LTB4-R and LTB4-S, tandem mass tag (TMT)-based quantitative proteomics method was adopted.

ORGANISM(S): Edwardsiella Tarda

SUBMITTER: Peng Bo

PROVIDER: PXD042752 | iProX | Tue Jun 06 00:00:00 BST 2023

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Publications

Functional Proteomics Analysis of Norfloxacin-Resistant Edwardsiella tarda.

Wu Jia-Han JH Li De-Li DL Tan Xiao-Hua XH Chen Xuan-Wei XW Liu Ying-Li YL Munang'andu Hetron Mweemba HM Peng Bo B

Journal of proteome research 20231019 11

Multidrug-resistant Edwardsiella tarda threatens both sustainable aquaculture and human health, but the control measure is still lacking. In this study, we adopted functional proteomics to investigate the molecular mechanism underlying norfloxacin (NOR) resistance in E. tarda. We found that E. tarda had a global proteomic shift upon acquisition of NOR resistance, featured with increased expression of siderophore biosynthesis and Fe3+-hydroxamate transport. Thus, e ...[more]

PMID: 37856871

Similar Datasets

Proteomic response of Escherichia coli to polyionene 6,6

Project description:This project contributes to the proteomic comparison of the dynamics of Escherichia coli O107 grown in TSB without polyionene 6,6 (PI 6,6) and with PI 6,6 either at 0.5 x Minimum Inhibitory Concentration (MIC) or 0.75 x MIC.

2025-09-15 | PXD056732 | Pride

proteome of oxytetracycline-resistant Edwardsiella tarda

Project description:We obtained a strain of oxytetracycline-resistant Edwardsiella tarda named LTB4-ROTC through artificial passaging in the TSB medium，the minimum inhibitory concentration (MIC) of LTB4-ROTC was 12.5 μg/mL, which was 32 folds increase contrast to LTB4-S strain. Proteomic analysis was conducted to profile the different protein expression.

2024-01-06 | PXD048431 |

Molecular signatures of the Eagle effect induced by the artificial siderophore conjugate LP-600 in E. coli

Project description:We investigated a paradoxical re-growth of E. coli upon treatment of a novel siderophore-conjugate, LP-600, at concentrations 16-32 times above the minimum inhibitory concentration (MIC).Transcriptome analysis revealed that LP-600 induced the expression of genes involved in SOS response and e14 prophage upon regrowth conditions.

2022-07-31 | GSE197880 | GEO

Comparative proteomics demonstrates altered Metabolism pathways in cotrimoxazole-resistant and amikacin-resistant Klebsiella pneumoniae isolates

Project description:The emergence and spread of carbapenem-resistant Klebsiella pneumoniae (CR-KPN) infections have worsened the current situation worldwide. Clinically, cotrimoxazole (CTX) and amikacin (AMI) are considered to be the preferred drugs in the treatment of (CR-KPN). But for now, the extensive use of cotrimoxazole (CTX) and amikacin (AMI) During the course of treatment leads to the emergence of cotrimoxazole- and amikacin-resistant infections, which is of great clinical concern. Previous evidence has shown that bacteria with reduced metabolism tend to be resistant to antibiotics, however, the mechanism remains unclear. In the present study, proteomics was performed on the sensitive, cotrimoxazole-resistant, amikacin-resistant and cotrimoxazole/amikacin-both-resistant KPN clinical isolates, and 2266 proteins were identified in total by liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) analysis. Further bioinformatic analysis showed down-regulation of tricarboxylic acid cycle pathway and up-regulation of alcohol metabolic or glutathione metabolism processes, which may contribute to ROS clearance and cell survival, in drug-resistant isolates. Finally, combined with minimum inhibitory concentration (MIC) of Amikacin and Cotrimoxazole on different KPN isolates, we identified nine proteins contributed mostly to such an alteration and the survival of bacteria under drug pressure, which could reveal novel mechanisms or pathways involved in drug resistance. These proteins and their pathways might be used as targets for the development of novel therapeutics against antimicrobial-resistant (AMR) infections.

2021-11-25 | PXD028544 | Pride

Sub-inhibitory concentrations of oxacillin modulate biogenesis and function of extracellular vesicles secreted by oxacillin-sensitive methicillin-resistant Staphylococcus aureus

Project description:Extracellular vesicles (EVs) derived from Staphylococcus aureus (S. aureus) carry multiple components, such as toxins, antigens, and resistance determinants, whose production is influenced by exposure to β-lactam antibiotics. However, systematic studies on the effects of β-lactam antibiotics on the release and functions of EVs remain limited. In this study, we isolated EVs from an OS-MRSA (OS-200) strain by ultracentrifugation, including both EVs untreated with antibiotics and those exposed to sub-inhibitory concentrations of oxacillin (OI-EVs). Exposure to sub-inhibitory concentrations of oxacillin (half and one-eighth of the minimum inhibitory concentration [MIC]) significantly enhanced the secretion of OI-EVs and increased the abundance of several proteins, including penicillin-binding protein 2, EmrB, Hlg, and enolase, in a concentration-dependent manner. Notably, EV1/2MIC exhibited more pronounced functional changes and enhanced hemolytic activity against rabbit red blood cells and promoted biofilm formation by OS-200. Additionally, OI-EVs stimulated the secretion of interleukin-6 and tumor necrosis factor-α by THP-1 macrophages in a dose-dependent manner and demonstrated greater penetration potential into A549 lung epithelial cells. Exposure to sub-inhibitory concentrations of oxacillin significantly altered the secretion and composition of EVs, highlighting a novel relationship between antimicrobial exposure, exosome biogenesis, and OS-MRSA pathogenicity. These findings provide new insights into how β-lactam antibiotics influence host–pathogen interactions by modulating the secretion of bacterial vesicles.

2025-07-22 | PXD066440 |

TraDIS-Xpress analysis of colistin stress responses in Escherichia coli

Project description:In past, resistance mechanisms have been identified by analysis of resistant isolates or defined mutants. Recently, high-throughput transposon mutagenesis coupled with sequencing (TraDIS-Xpress) is another approach proving useful for elucidating the roles of genes involved in the overall cellular response to a particular stress. In this study, we used TraDIS-Xpress to determine the role played by genes following exposure to colistin stress. Approximately 10^7 cells from the mutant library were inoculated into LB broth at a range of doubling concentrations of colistin ( 0.25 x MIC, 0.5 x MIC, 1 x MIC, 2 X MIC). Experiments were performed with no induction, or with induction using 0.2 or 1 mM of Isopropyl β-D-1-thiogalactopyranoside (IPTG). All experiments were performed in duplicate.

2022-06-09 | E-MTAB-11807 | biostudies-arrayexpress

The transcriptional response of Pasteurella multocida to three classes of antibiotics

Project description:We studied the effects of three classes of antibiotics (amoxicillin, chlortetracycline and enrofloxacin ) on P. multocida transcriptome using custom oligonucleotide microarrays from Nimblegen systems. All the 2015 genes of Pm70 were spotted on the array and hybridizations were carried out with RNA isolated from three independent cultures of Pm70 grown in the presence or absence of sub-minimum inhibitory (sub-MIC) doses of antibiotics. Differentially expressed genes were identified by ANOVA and Dunnett’s test. Biological modeling of the differentially expressed genes (DE) was carried out based on Clusters of Orthologous (COG) groups and network analysis in Pathway Studio. Keywords: Response to sub-MIC antibiotics

2009-09-01 | GSE12779 | GEO

Profiling the effect of Nafcillin on HA-MRSA D712 using bacteriological and physiological media

Project description:Vancomycin Intermediate Staphylococcus aureus (VISA) strain originated during the prolonged hospitalization, chronic infection and vancomycin treatment. The hospital acquired D712 VISA strain were subjected to various sub-inhibitory concentration of nafcillin in Cation Adjusted Mueller Hinton Broth (CA-MHB) and Roswell Park Memorial Institute (RPMI) medium. The MIC of has dropped from 256 ug/ml to 1ug/ml in CA-MHB and RPMI media respectively. The cells were harvested and expression profiling were measured using RNA sequencing at time point of 03 hours.

2019-10-22 | GSE139141 | GEO

Analysis of antibiotic response in Clinical Wound Pseudomonas aeruginosa isolates: Unveiling Proteome Dynamics of tobramycin tolerant phenotype

Project description:Pseudomonas aeruginosa (PA) is an opportunistic human pathogen, causing serious chronic infections. PA can adapt efficiently to antibiotic stressors via different genotypic or phenotypic strategies such as resistance and tolerance. The adaptation regulatory system is not always very well understood. In this study, we use shotgun proteomics to investigate the system-level response to tobramycin in two clinical wound PA isolates and PAO1. We profiled each strain for its antibiotic drug-tolerant phenotype using supra-minimum inhibitory concentrations (supra-MIC) of tobramycin and apply proteomics to investigate the protein expression profiles. The MIC revealed that all isolates were susceptible to tobramycin but at supra-MIC concentrations at stationary growth, a degree of tolerance was observed for the isolates. We identified around 40 % of the total proteins encoded by the PA genome and highlighted shared and unique protein signatures for all isolates. Comparative proteome profiling in the absence of antibiotic treatment showed divergent fingerprints, despite similarities in the growth behavior of the isolates. In the presence of tobramycin, the isolates shared a common response in the downregulation of proteins involved in the two-component system, whereas stress response proteins were present at higher levels. Our findings provide insight into the use of proteomic tools to dissect the system-level response in clinical isolates in the absence and presence of antibiotic stress.

2024-10-17 | PXD051528 | Pride

Transcriptomes of Enterococcus faecalis OG1RF and OG1RF Δbph in planktonic culture

Project description:Liquid cultures of Enterococcus faecalis OG1RF and OG1RF Δbph were grown in tryptic soy broth without added dextrose (TSB-D) for 2 and 4 hr. At each time point, the transcriptomes were compared to identify differentially expressed genes in the Δbph mutant.

2022-03-12 | GSE198051 | GEO