Proteomics

Dataset Information

0

HEK cells expressing miRFP680-P2A-GFP, emiRFP670-P2A-GFP, emiRFP703-P2A-GFP, miRFP713-P2A-GFP and miRFP720-P2A-GFP

ABSTRACT: HEK cells transiently transfected with pAAV-CAG-NIR-FPs-P2A-GFP plasmids were processed using Mini MS Sample Prep kit according to the manufacture protocol. Extracted peptides were analyzed using Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher) in data-independent acquisition (DIA) mode. We reported the protein identity and relative concentration of NIR FPs and GFPs for each analyzed sample as well as post-translational modifications if any are identified.

ORGANISM(S): Homo Sapiens

SUBMITTER: Kiryl Piatkevich  

PROVIDER: PXD042781 | iProX | Wed Jun 07 00:00:00 BST 2023

REPOSITORIES: iProX

Json Xml
altmetric image

Publications

Recent progress in fluorescent protein development has generated a large diversity of near-infrared fluorescent proteins (NIR FPs), which are rapidly becoming popular probes for a variety of imaging applications. However, the diversity of NIR FPs poses a challenge for end-users in choosing the optimal one for a given application. Here we conducted a systematic and quantitative assessment of intracellular brightness, photostability, oligomeric state, chemical stability and cytotoxicity of 22 NIR  ...[more]

Similar Datasets

Transcriptomics Gene expression of HEK 293 cells transfected with Vector (PCMV), Aire, or MBD-VP16
2013-11-21 | E-GEOD-52546 | biostudies-arrayexpress
Transcriptomics Gene expression of HEK 293 cells transfected with Vector (PCMV), Aire, or MBD-VP16
2013-11-21 | GSE52546 | GEO
Transcriptomics Complexity of expression from transiently transfected plasmids
2012-08-17 | E-GEOD-36062 | biostudies-arrayexpress
Transcriptomics Complement Membrane Attack Complexes Disrupt Proteostasis to Function as Intracellular Alarmins
2024-06-17 | GSE268767 | GEO
Transcriptomics Complexity of expression from transiently transfected plasmids
2012-08-17 | GSE36062 | GEO
Transcriptomics Mechanism of virus attenuation by codon pair deoptimization
2020-01-01 | GSE134752 | GEO
Genomics Homo sapiens
| PRJNA151813 | ENA
Proteomics EXTL3ΔN purification from EXT1 CRISPR HEK
2022-07-12 | PXD032144 | Pride
Proteomics samon_1803_DIA_DDA_HEK - Sensitive quantitative proteomics of human hematopoietic stem and progenitor cells by data-independent acquisition mass spectrometry
2019-04-25 | PXD009246 | Pride
Transcriptomics RNAseq analysis of whole Guts over expressing LaminDm0 or GFP in Enterocytes
2019-07-15 | GSE112640 | GEO