Potential role of lysine acetylation in brown film formation and post-ripening of Lentinula edodes mycelium

ABSTRACT: Lentinula edodes is one of the most widely cultivated edible mushrooms in the world. When cultivated in sawdust, the surface mycelium of L. edodes needs a long post-ripening stage wherein it forms a brown film (BF) by secreting and accumulating pigments. BF formation is critical for the high quality and yield of fruiting bodies. Protein lysine acetylation (KAC) is an important post-translational modification that regulates growth and development. Previous studies have shown that deacetylase levels are significantly increased during BF formation in the post-ripening stage of L. edodes. The aim of this study was to assess the role of protein acetylation during BF formation. To this end, we compared the proteomes and acetylomes of L. edodes mycelia before and after BF formation using label-free quantitative proteomics, acetylation enrichment techniques and high-resolution liquid chromatography-mass spectrometry-based quantitative proteomics. We identified 5613 acetylation sites in 1991 proteins, and quantitative information was available for 4848 of these sites in 1815 proteins. Comparative acetylome analysis showed that the modification of 699 sites increased and that of 562 sites decreased during BF formation. Bioinformatics analysis of the differentially acetylated proteins showed significant enrichment in the tricarboxylic acid (TCA) cycle and proteasome pathways. Furthermore, functional assays showed that BF formation is associated with significant changes in the activities of proteasome, citrate synthase and isocitrate dehydrogenase, indicating that the acetylation of proteasomal and TCA cycle proteins are critical for BF formation and post-ripening. Consistent with this hypothesis, the lysine deacetylase inhibitor trichostatin (TSA) delayed autophagy and BF formation in L. edodes. Taken together, KAC and autophagy play important roles in the mycelial BF formation and post-ripening stage of L. edodes.

ORGANISM(S): Lentinula Edodes

SUBMITTER: Dapeng Bao  

PROVIDER: PXD042811 | iProX | Thu Jun 08 00:00:00 BST 2023

REPOSITORIES: iProX