2-DE based proteomics of spleen
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus Musculus
SUBMITTER: 
Bo Peng
PROVIDER: PXD042893 | iProX | Mon Jun 12 00:00:00 BST 2023
REPOSITORIES: iProX
Similar Datasets
Project description:<p>Multidrug-resistant bacteria present a major threat to public health. Therefore, new drugs or approaches are urgently needed to manage and mitigate this threat. Here, we screen the molecular candidates that allow the survival of mice upon <em>Vibrio parahaemolyticus</em> infection by integrated proteomic and metabolomics analysis, where L-Alanine metabolism and phagocytosis are highly correlated. The role of L-Alanine on boosting mouse survival is further confirmed with <em>in vivo</em> bacterial challenge studies on bacteria including <em>V. parahaemolyticus</em>, <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em> and <em>Klebsiella pneumoniae</em>. Functional studies demonstrate that exogenous L-Alanine promotes phagocytosis to these different species of multidrug-resistant pathogens. The underlying mechanism involves two events that are L-Alanine-dependently increased TLR4 expression and L-Alanine-enhanced TLR4 signaling via increasing the biosynthesis and secretion of fatty acids such as palmitate. Palmitate enhances the binding of LPS to TLR4 and thereby promotes TLR4 dimmer formation and endocytosis for the subsequent activation of PI3K/Akt and NF-κB pathways and phagocytosis of bacteria. These results suggest that modulation of metabolic environment is a plausible approach for combating infection with multidrug-resistant bacteria.</p><p><br></p><p><strong>V. parahaemolyticus-infected spleen metabolomics</strong> is reported in the current study <a href='https://www.ebi.ac.uk/metabolights/MTBLS7996' rel='noopener noreferrer' target='_blank'><strong>MTBLS7996</strong></a>.</p><p><strong>L-Alanine-treated macrophage metabolomics</strong> is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS7986' rel='noopener noreferrer' target='_blank'><strong>MTBLS7986</strong></a>.</p>
2024-09-26 | MTBLS7996 | MetaboLights
Project description:<p>Multidrug-resistant bacteria present a major threat to public health. Therefore, new drugs or approaches are urgently needed to manage and mitigate this threat. Here, we screen the molecular candidates that allow the survival of mice upon <em>Vibrio parahaemolyticus</em> infection by integrated proteomic and metabolomics analysis, where L-Alanine metabolism and phagocytosis are highly correlated. The role of L-Alanine on boosting mouse survival is further confirmed with <em>in vivo</em> bacterial challenge studies on bacteria including <em>V. parahaemolyticus</em>, <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em> and <em>Klebsiella pneumoniae</em>. Functional studies demonstrate that exogenous L-Alanine promotes phagocytosis to these different species of multidrug-resistant pathogens. The underlying mechanism involves two events that are L-Alanine-dependently increased TLR4 expression and L-Alanine-enhanced TLR4 signaling via increasing the biosynthesis and secretion of fatty acids such as palmitate. Palmitate enhances the binding of LPS to TLR4 and thereby promotes TLR4 dimmer formation and endocytosis for the subsequent activation of PI3K/Akt and NF-κB pathways and phagocytosis of bacteria. These results suggest that modulation of metabolic environment is a plausible approach for combating infection with multidrug-resistant bacteria.</p><p><br></p><p><strong>L-Alanine-treated macrophage metabolomics</strong> is reported in the current study <a href='https://www.ebi.ac.uk/metabolights/MTBLS7986' rel='noopener noreferrer' target='_blank'><strong>MTBLS7986</strong></a>.</p><p><strong>V. parahaemolyticus-infected</strong> <strong>spleen metabolomics </strong>is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS7996' rel='noopener noreferrer' target='_blank'><strong>MTBLS7996</strong></a>.</p>
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Project description:Ectromelia virus (ECTV) has emerged as a valuable model for investigating the host-orthopoxvirus relationship as it relates to pathogenesis and the immune response. ECTV causes mousepox in most strains of mice, including BALB/c and DBA/2, and these are therefore classified as susceptible mice. Conversely, C57BL/6 and certain 129 strains display limited pathology and a very low mortality, and are thus classified as resistant. To understand the host genetic factors of different mouse strains in response to ECTV infection, we carried out a microarray analysis using Affymetrix Gene-Chip Mouse Genome Arrays of spleen tissues from BALB/c and C57BL/6 mice at 3 and 10 days post-ECTV infection. Differential Expression of Genes (DEGs) analyses revealed distinct differences in the gene profiles of resistant and susceptible mice infected with ECTV. Gene ontology and KEGG pathway analysis showed that the DEGs of susceptible mice were involved mainly in immunity, apoptosis, spliceosomes and cancer-related pathways, while the DEGs of resistant mice were largely involved in MAPK signaling and leukocyte transendothelial migration. This suggests that the susceptible BALB/c mice have a stronger response than the resistant C57BL/6 mice to ECTV infection. The BALB/c mice showed a strong induction of interferon-induced genes (ISGs), including guanylate binding proteins (GBPs), myxovirus resistance protein (Mx) GTPases, oligoadenylate synthetase (OAS) and IFN-induced protein with tetratricopeptide repeats (IFIT) family proteins, while the C57BL/6 mice upregulated more genes related to metabolic pathways. This suggests that the susceptible BALB/c mice have a stronger response than the resistant C57BL/6 mice to ECTV infection.
2018-02-16 | GSE100644 | GEO
Project description:Vibrio parahaemolyticus is the leading bacterial cause for seafood-related gastroenteritis worldwide. As an intestinal pathogen, V. parahaemolyticus competes with other commensal bacteria for the same pool of nutrients. The major source of nutrition for intestinal bacteria is intestinal mucus. We wanted to determine the expression profile of wild-type V. parahaemolyticus in mouse intestinal mucus and then perform a differential expression analysis in a ∆rpoN deletion mutant.
2020-02-14 | GSE95555 | GEO
Project description:This is a time course study of differential gene expression in the thymus and spleen of susceptible (Balb/c) and resistant (C57BL/6) mice during experimental Trypanosoma cruzi infection. There is a special interest in the expression of galectins, glycosyltransferases, neuraminidases and chemokines in the gene expression patterns. Mice were infected with 1000 trypomastigotes of T. cruzi strain Y i.p. Organs (thymus and spleen) were removed aseptically at days 0, 7, 14, 21 and 28 post-infection. The time points have been selected as they allow the following of the acute phase of the disease with the peak of parasitemia (between 7 and 14 days), and the chronic phase (more than 21 days post infection). RNA from at least three animals from the same data point was pooled to normalize internal variability. Total RNA was isolated as per recommended protocol and shipped to the Core E site. Samples were hybridized to the GLYCOv3 array.
2011-04-29 | E-GEOD-28921 | biostudies-arrayexpress
Project description:bacteriophages targeting multidrug-resistant bacteria
| PRJEB83222 | ENA
Project description:Vibrio parahaemolyticus is the leading bacterial cause for seafood-related gastroenteritis worldwide. As an intestinal pathogen, V. parahaemolyticus competes with other commensal bacteria for the same pool of nutrients. The major source of nutrition for intestinal bacteria is intestinal mucus. We wanted to determine the expression profile of wild-type V. parahaemolyticus in mouse intestinal mucus and then perform a differential expression analysis in a ∆luxO deletion mutant, in which the high cell density quorum sensing regulator OpaR is constitutively expressed and low cell density regulator AphA is repressed.
2017-01-13 | GSE93547 | GEO