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Quantitative proteomics investigation of total protein and acetylated 4D Label free of 18 samples from H9C2 cells

ABSTRACT: The quantitative investigation of acetylated proteomics was carried out in this project. To ensure high reliability of the results, we filtered the identification data using a criterion of localization probability>0.75. This study identified a total of 9755 acetylation sites located on 3051 proteins, of which the quantitative information from 6323 sites of 2005 proteins were obtained. Subsequently, we conducted a systematic bioinformatics analysis of proteins which containing quantitative information sites, including protein annotation, functional classification, functional enrichment, and cluster analysis based on functional enrichment. According to above information, we provided reference directions for downstream in-depth research based on proteomics.

ORGANISM(S): Rattus Norvegicus

SUBMITTER: Xiangyan Li

PROVIDER: PXD043035 | iProX | Fri Jun 16 00:00:00 BST 2023

REPOSITORIES: iProX

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Ginsenoside Rb2 inhibits p300-mediated SF3A2 acetylation at lysine 10 to promote Fscn1 alternative splicing against myocardial ischemic/reperfusion injury.

Huang Qingxia Q Yao Yao Y Wang Yisa Y Li Jing J Chen Jinjin J Wu Mingxia M Guo Chen C Lou Jia J Yang Wenzhi W Zhao Linhua L Tong Xiaolin X Zhao Daqing D Li Xiangyan X

Journal of advanced research 20231213

<h4>Introduction</h4>Ginsenosides (GS) derived from Panax ginseng can regulate protein acetylation to promote mitochondrial function for protecting cardiomyocytes. However, the potential mechanisms of GS for regulating acetylation modification are not yet clear.<h4>Objectives</h4>This study aimed to explore the potential mechanisms of GS in regulating protein acetylation and identify ginsenoside monomer for fighting myocardial ischemia-related diseases.<h4>Methods</h4>The 4D-lable free acetylomi ...[more]

PMID: 38101749

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