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Systematic discovery of linear ubiquitination mediated neo-protein-protein interactions across mouse tissues

ABSTRACT: HOIP truncations proteins GST-PUB, GST-NZF, GST-UBA and GST-RBR-LDD were purified, GST was used as a negative control. The tissue lysates from the C57BL/6 mice were incubated with glutathione-S-transferase (GST) beads which bound to the GST-HOIP truncation proteins beforehand. The pull-down component were separated by SDS-PAGE and stained with Coomassie brilliant blue. The band were cut off and subject to mass spectrometry.

ORGANISM(S): Mus Musculus

SUBMITTER: Lingqiang Zhang

PROVIDER: PXD043322 | iProX | Wed Feb 28 00:00:00 GMT 2024

REPOSITORIES: iProX

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Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis.

Fu Yesheng Y Li Lei L Zhang Xin X Deng Zhikang Z Wu Ying Y Chen Wenzhe W Liu Yuchen Y He Shan S Wang Jian J Xie Yuping Y Tu Zhiwei Z Lyu Yadi Y Wei Yange Y Wang Shujie S Cui Chun-Ping CP Liu Cui Hua CH Zhang Lingqiang L

Nature communications 20240406 1

Linear ubiquitination catalyzed by HOIL-1-interacting protein (HOIP), the key component of the linear ubiquitination assembly complex, plays fundamental roles in tissue homeostasis by executing domain-specific regulatory functions. However, a proteome-wide analysis of the domain-specific interactome of HOIP across tissues is lacking. Here, we present a comprehensive mass spectrometry-based interactome profiling of four HOIP domains in nine mouse tissues. The interaction dataset provides a high-q ...[more]

PMID: 38582895

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