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1 / 2 MIC Loratadine and DMSO control group of Streptococcus agalactiae quantitative proteomics to observe the global expression of protein in DMSO treatment group and Loratadine group

ABSTRACT: Streptococcus agalaciate isolate was inoculated and cultured to logarithmic growth phase (OD600 at 0.5) in TSB and divided into two groups, the control group and the drug treated group. Loratadine was added to the drug treated group until a final concentration of 1/2MIC reached, and DMSO added in the control group. Then, cultures in both groups were cultivated for 2h. The microbial was centrifuged at 12000 rpm for 10 min, washed twice with Phosphate Buffer Saline (PBS). Lyse the cells by bead-beating at 4 °C in RIPA lysis buffer (1% Triton X-100, 1% deoxycholate, 0.1% SDS), The protein reduction, alkylation and tryptic digestion were followed as filter-aided sample preparation (FASP). The digestion products were analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), followed by label-free quantitative analysis. The MS experiment was performed on a Q Exactive plus mass spectrometer.

ORGANISM(S): Streptococcus Agalactiae Atcc 13813

SUBMITTER: Zewen Wen

PROVIDER: PXD043548 | iProX | Wed Jul 05 00:00:00 BST 2023

REPOSITORIES: iProX

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