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Proteomic analysis of storage properties of Lacticaseibacillus paracasei Zhang starter culture by DNA methylation

ABSTRACT: This study took the advantage of the availability of a methylated mutant (ΔpglX ) of Lacticaseibacillus (L.) paracasei Zhang to test our hypothesis that DNA methylation could protect lactic acid bacteria from freeze-drying and post-freeze-drying storage stresses. Active cultures of the wild-type and mutant were freeze-dried and stored at 30 ℃ for different duration (30, 60, and 90 days) before reactivation. Proteomic analyses was implemented to identify differentially expressed proteins (DEPs) of the bacteria.

ORGANISM(S): Lactobacillus Paracasei

SUBMITTER: Wenyi Zhang

PROVIDER: PXD043736 | iProX | Wed Jul 12 00:00:00 BST 2023

REPOSITORIES: iProX

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Abrogating the adenine methylation ability of Lacticaseibacillus paracasei improves its freeze-drying and storage resistance.

Qiao Hui H You Mingkun M Yan Jiaming J Zhang Meng M Lai-Yu Kwok K Zhang Wenyi W

NPJ science of food 20250519 1

Freeze-drying is a widely adopted method for the long-term storage of starter cultures in the food industry but can cause cell instability and a decline in post-storage viability. We used an unmethylated Lacticaseibacillus paracasei Zhang mutant lacking adenine-specific DNA-methyltransferase. This mutant was subjected to freeze-drying and stored at 30 °C for two distinct durations (30 and 60 days), Our analysis revealed the unmethylated mutant outperformed the wild-type in cell viability and sur ...[more]

PMID: 40389488

Similar Datasets

PRM analysis of storage properties of Lacticaseibacillus paracasei Zhang starter culture by DNA methylation

Project description:This study took the advantage of the availability of a methylated mutant (ΔpglX ) of Lacticaseibacillus (L.) paracasei Zhang to test our hypothesis that DNA methylation could protect lactic acid bacteria from freeze-drying and post-freeze-drying storage stresses. Active cultures of the wild-type and mutant were freeze-dried and stored at 30 ℃ for different duration (30, 60, and 90 days) before reactivation. Proteomic analyses was implemented to identify differentially expressed proteins (DEPs) of the bacteria.The confidence of proteomic results was assessed by PRM validation.

2023-07-12 | PXD043735 |

PRM analyses of Lacticaseibacillus paracasei Zhang under ethanol stress

Project description:This study hypothesized that adenine methylation was involved in bacterial regulation of physiological adaptations when grown under ethanol stress. Thus, this study compared the growth of an adenine methyltransferase-inactivated mutant (ΔpglX) and the wild-type L. paracasei Zhang strains when cultured under ethanol stress. Proteomic analyses was implemented to identify differentially expressed proteins (DEPs) of the bacteria.

2022-12-05 | PXD038534 |

Proteomic analyses of Lacticaseibacillus paracasei Zhang under ethanol stress

2022-08-17 | PXD036137 |

PRM analyses of Lacticaseibacillus paracasei Zhang under osmotic stress

Project description:This study hypothesized that adenine methylation was involved in bacterial regulation of physiological adaptations when grown under environment stress. Thus, this study compared the growth of an adenine methyltransferase-inactivated mutant (ΔpglX) and the wild-type L. paracasei Zhang strains when cultured under osmotic stress. Proteomic analyses were implemented to identify differentially expressed proteins (DEPs) of the bacteria under osmotic stress .

2022-12-05 | PXD038537 |

Proteomic analyses of Lacticaseibacillus paracasei Zhang under osmotic stress

2022-08-17 | PXD036138 |

Lacticaseibacillus paracasei strain:Zhang

Project description:Lacticaseibacillus paracasei strain:Zhang Genome sequencing

| PRJNA28537 | ENA

Differences in Acid Stress Response of Lacticaseibacillus paracasei Zhang Cultured from Solid-State Fermentation and Liquid-State Fermentation.

Project description:Liquid-state fermentation (LSF) and solid-state fermentation (SSF) are two forms of industrial production of lactic acid bacteria (LAB). The choice of two fermentations for LAB production has drawn wide concern. In this study, the tolerance of bacteria produced by the two fermentation methods to acid stress was compared, and the reasons for the tolerance differences were analyzed at the physiological and transcriptional levels. The survival rate of the bacterial agent obtained from solid-state fermentation was significantly higher than that of bacteria obtained from liquid-state fermentation after spray drying and cold air drying. However, the tolerance of bacterial cells obtained from liquid-state fermentation to acid stress was significantly higher than that from solid-state fermentation. The analysis at physiological level indicated that under acid stress, cells from liquid-state fermentation displayed a more solid and complete membrane structure, higher cell membrane saturated fatty acid, more stable intracellular pH, and more stable activity of ATPase and glutathione reductase, compared with cells from solid-state fermentation, and these physiological differences led to better tolerance to acid stress. In addition, transcriptomic analysis showed that in the cells cultured from liquid-state fermentation, the genes related to glycolysis, inositol phosphate metabolism, and carbohydrate transport were down-regulated, whereas the genes related to fatty acid synthesis and glutamate metabolism were upregulated, compared with those in cells from solid-state fermentation. In addition, some genes related to acid stress response such as cspA, rimP, rbfA, mazF, and nagB were up-regulated. These findings provide a new perspective for the study of acid stress tolerance of L. paracasei Zhang and offer a reference for the selection of fermentation methods of LAB production.

| S-EPMC8465097 | biostudies-literature

Transcriptomic analysis of <i>Lacticaseibacillus paracasei</i> Zhang in transition to the viable but non-culturable state by RNA sequencing.

Project description:BackgroundSome bacteria enter the viable but non-culturable (VBNC) state to survive harsh environmental conditions and external stresses. This alters cell physiology and has implications for the food industry as some bacteria, such as lactobacilli, undergo similar changes during food processing.MethodsThis study aimed to investigate the transcriptomic changes of a probiotic strain, Lacticaseibacillus paracasei Zhang (L. paracasei Zhang), upon transition to the VBNC state using high throughput RNA sequencing (RNA-seq).ResultsBacteria were inoculated into the de Man, Rogosa, and Sharpe medium and maintained at low temperature and pH to induce cell transition to the VBNC state. Cells were harvested for analysis at five stages of VBNC induction: 0, 3, 30, and 180 days after induction and 210 days when the cells entered the VBNC state. Our results showed that the expression of 2,617, 2,642, 2,577, 2,829, and 2,840 genes was altered at these five different stages. The function of differentially expressed genes (DEGs, compared to healthy cells collected at day 0) and their encoded pathways were analyzed by the Gene Ontology Consortium and the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. A total of 10 DEGs were identified in cells that entered the VBNC state: five continuously upregulated (LCAZH_0621, LCAZH_1986, LCAZH_2038, LCAZH_2040, and LCAZH_2174) and five continuously downregulated (LCAZH_0024, LCAZH_0210, LCAZH_0339, LCAZH_0621, and LCAZH_0754).ConclusionsThis study proposes a molecular model of the VBNC mechanism in L. paracasei Zhang, highlighting that changes in cell metabolism improve substrate utilization efficiency, thereby enhancing bacterial survival under adverse conditions. These data may be useful for improving the survival of probiotics in industrial food processing.

| S-EPMC10768001 | biostudies-literature

Lacticaseibacillus paracasei derived Extracellular vesicles LC-MS/MS

Project description:Owing to their ability to secrete antimicrobials and benefit human health, lactic acid bacteria (LAB) cultures are an attractive nontoxic alternative to antibiotics for preserving food and combating pathogenic infections. Given that this strategy has several limitations, including strain-dependent antimicrobial effectiveness, reduced efficacy against multidrug-resistant strains, and difficulties in large-scale production without bacterial contamination, current research focuses on identifying and utilizing endolysins (enzymes that degrade bacterial cell walls) produced by novel or engineered LAB cultures to inhibit pathogen growth. The challenges faced by this approach (e.g., bactericidal activity lower than that of antibiotics, susceptibility to degradation by proteases, and high purification and quality control costs) can be overcome using engineered LAB-derived extracellular vesicles (LEVs) displaying pathogen-specific endolysins on their surface to directly recognize and eliminate target pathogens. Given that no LEV surface-displayed proteins (SDPs) have been characterized to date, this study identifies and characterizes a LEV SDP (LP-SDP3) from Lacticaseibacillus paracasei using proteomic analysis, heterologous expression of candidate SDPs, biochemical analysis, and SpyTag–SpyCatcher reactions. LP-SDP3 homologs are found in Escherichia coli and other LAB strains, exhibiting the same function in E. coli and Lactococcus lactis. Endolysin (PlyF307SQ-8C)-displaying LEVs derived from L. paracasei selectively kill Staphylococcus aureus, exhibiting an activity comparable with that of purified PlyF307SQ-8C. This study is the first to identify a universal extracellular vesicle SDP for E. coli and LAB strains, demonstrating its potential as a platform for developing endolysin–extracellular vesicle antibacterials without the need for labor-intensive and costly endolysin preparation.

2025-07-21 | PXD059432 | Pride

Complete Genome Sequence of Lacticaseibacillus paracasei Strain NSMJ15.

Project description:We report here the complete genome sequence of Lacticaseibacillus paracasei NSMJ15, isolated from makgeolli (a traditional Korean fermented liquor) and shown to have potentially probiotic characteristics. The genome consisted of a 2.79-Mbp chromosome contig and four plasmids having a total of 2,947 genes, including 2,690 coding sequences.

| S-EPMC8656385 | biostudies-literature

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