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Raw data of mass spectrometry -Jisheng Li

ABSTRACT: Using CXMS to probe the effect of persulfidation on AtG6PD6 homooligomer structure. Abundant cross-links were identified within both AtG6PD6 monomer and oligomer (Datasets S2 and 3). Based on the cross-linking information, models of the AtG6PD6 dimer and tetramer structures were constructed.

ORGANISM(S): Arabidopsis Thaliana

SUBMITTER: Jisheng Li

PROVIDER: PXD043969 | iProX | Thu Jul 20 00:00:00 BST 2023

REPOSITORIES: iProX

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Publications

Persulfidation maintains cytosolic G6PDs activity through changing tetrameric structure and competing cysteine sulfur oxidation under salt stress in Arabidopsis and tomato.

Wang Xiaofeng X Shi Cong C Hu Yanfeng Y Ma Ying Y Yi Yuying Y Jia Honglei H Li Fali F Sun Haotian H Li Tian T Wang Xiuyu X Li Tianjinhong T Li Jisheng J

The New phytologist 20230813 2

Glucose-6-phosphate dehydrogenases (G6PDs) are essential regulators of cellular redox. Hydrogen sulfide (H<sub>2</sub> S) is a small gasotransmitter that improves plant adaptation to stress; however, its role in regulating G6PD oligomerization to resist oxidative stress remains unknown in plants. Persulfidation of cytosolic G6PDs was analyzed by mass spectrometry (MS). The structural change model of AtG6PD6 homooligomer was built by chemical cross-linking coupled with mass spectrometry (CXMS). W ...[more]

PMID: 37574819

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