Systematic affinity purification mass spectrometry (AP-MS) reveals LCMV interacted human proteins

ABSTRACT: To systematically map the arenavirus‒host protein interaction, we employed a systematic affinity purification mass spectrometry (AP-MS) approach to define the human proteins that physically interact with LCMV proteins. The four LCMV proteins (NP, LP, GPC, Z) and control protein GFP were tandemly cloned with Twin-Strep tags at C-terminal based on the same backbone pCAGGs vector respectively. Proteins were expressed in HEK293T cells followed by affinity-purification based on Strep-Tactin System. We summarized the number of human proteins interacting with each indicated LCMV protein baits and identified 284 human proteins with high-confidence PPIs (log2 enriched fold > 1, P < 0.05).

ORGANISM(S): Homo Sapiens

SUBMITTER: Leike Zhang  

PROVIDER: PXD044162 | iProX | Fri Jul 28 00:00:00 BST 2023

REPOSITORIES: iProX