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IBT quantitative Proteomics of TEKTIP1-KO and wild mouse sperm explore the functional defect caused by Tektip1 deletion

ABSTRACT: First, we constructed knockout mice of tektip1 using gene-editing technology. In order to explore how tektip1 gene knockout leads to structural defects, we plan to use IBT quantitative proteomics to find out the differential expression of proteins, providing ideas for exploring the defects caused by tektip1 knockout.

ORGANISM(S): Mus Musculus

SUBMITTER: Suren Chen

PROVIDER: PXD044492 | iProX | Wed Aug 09 00:00:00 BST 2023

REPOSITORIES: iProX

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Tektin bundle interacting protein, TEKTIP1, functions to stabilize the tektin bundle and axoneme in mouse sperm flagella.

Geng Xin-Yan XY Jin Hui-Juan HJ Xia Lan L Wang Bin-Bin BB Chen Su-Ren SR

Cellular and molecular life sciences : CMLS 20240307 1

Tektins are microtubule inner proteins (MIPs) and localize at the inside lumen of doublet microtubules (DMTs) of cilia/flagella. TEKTIP1, a newly identified protein by cryo-electron microscopy (cryo-EM), is proposed to be localized at the center of the tektin bundle and hypothesized to recruit tektins or stabilize the bundle. However, the physiological role of TEKTIP1 is unknown. In this study, we generated Tektip1-knockout (Tektip1<sup>-/-</sup>) mice and showed that they were male subfertile p ...[more]

PMID: 38448737

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