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LFQ quantitative analysis of 6 rhesus macaques prefrontal cortex samples from Shanghai Ninth People's Hospital

ABSTRACT: Rhesus macaques that were exposed to sevoflurane on three occasions during the early postnatal period (days 7, 21, and 35) showed signs of anxiety and impaired visual recognition memory, according to previous studies. By using this animal model, we were able to study the changes in proteomics and RNA sequencing in the prefrontal cortex of rhesus macaques after sevoflurane anesthesia.Proteomics analysis of the brain found that the functions of different proteins expressed after sevoflurane exposure in rhesus macaques were mainly related to mitochondrial respiration.

ORGANISM(S): Macaca Mulatta

SUBMITTER: Lei Zhang

PROVIDER: PXD044509 | iProX | Thu Aug 10 00:00:00 BST 2023

REPOSITORIES: iProX

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Project description:Lassa fever (LF) is a rodent-borne viral disease that can be fatal for human beings. In this study, an attenuated Lassa vaccine candidate, ML29, was tested in SIV-infected rhesus macaques for its ability to elicit immune responses without instigating signs of virulent disease. ML29 is a reassortant between Lassa and Mopeia viruses that causes a transient infection in non-human primates and confers sterilizing protection from lethal Lassa viral challenge. However, since the LF endemic area of West Africa also has high HIV seroprevalence, it is important to determine whether vaccination could be safe in the context of AIDS. SIV-infected and uninfected rhesus macaques were vaccinated with the ML29 virus and monitored for classical and non-classical signs of arenavirus disease. Classical disease signs included viremia, rash, weight loss, high liver enzyme levels, and virus invasion of the central nervous system. Non-classical signs derived from profiling the blood transcriptome of virulent and non-virulent arenavirus infections included increased expression of interferon response genes and decreased expression of COX2, IL-1?, coagulation intermediates and nuclear receptors needed for stress signaling. Here it is demonstrated that SIV-infected and uninfected rhesus macaques responded similarly to ML29 vaccination, and that none developed signs of arenavirus disease or persistence. Furthermore, 5 of 5 animals given a heterologous challenge with a lethal dose of LCMV-WE survived without developing disease signs. 30 RNA samples from Monkey PBMC: 4 uninf. Monkey PBMC, 8 SIV-infected Monkey PBMC(From 8 Monkeys), 5 SIV+ML29-sc infected week1(Monkey PBMC), 5 SIV+ML29-sc infected week2(Monkey PBMC), 1 SIV+ML29-ig infected week1(Monkey PBMC), 1 SIV+ML29-ig infected week2(Monkey PBMC), 2 SIV+Arm-sc infected week1(Monkey PBMC), 2 SIV+Arm-sc infected week2(Monkey PBMC), 1 only ML29-iv infected week1(Monkey PBMC), 1 only ML29-iv infected week2(Monkey PBMC)

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LFQ quantitative analysis of 6 rhesus macaques prefrontal cortex samples from Shanghai Ninth People's Hospital

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