Proteomics

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TMT Labeling proteomic analysis of wild and mutant of OsGSTU34 transporter in black rice caryopsis


ABSTRACT: TMT labeling proteomic analysis was performed on black rice caryopsis after editing an important anthocyanin transporter gene called OsGSTU34

ORGANISM(S): Oryza Sativa

SUBMITTER: Piqing Liu  

PROVIDER: PXD044599 | iProX | Tue Aug 15 00:00:00 BST 2023

REPOSITORIES: iProX

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Publications

OsGSTU34, a Bz2-like anthocyanin-related glutathione transferase transporter, is essential for rice (Oryza sativa L.) organs coloration.

Mackon Enerand E   Guo Yongqiang Y   Jeazet Dongho Epse Mackon Guibeline Charlie GC   Ma Yafei Y   Yao Yuhang Y   Luo Dengjie D   Dai Xianggui X   Zhao Neng N   Lu Ying Y   Jandan Tahir Hussain TH   Liu Piqing P  

Phytochemistry 20231020


Anthocyanins are a flavonoid compound known as one of the most important chromogenic substances. They play several functions, including health promotion and sustaining plants during adverse conditions. They are synthesized at the endoplasmic reticulum and sequestered in the vacuole. In this work, we generated knock-out lines of OsGSTU34, a glutathione transporter's tau gene family, with no transgene line and off-target through CRISPR/Cas9 mutagenesis and highlighted the loss of pigmentation in r  ...[more]

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