Dataset Information

Quantitative proteomics reveals the protective mechanism of glutamine against enteritis in hybrid groupers

ABSTRACT: Five experimental diets were prepared with equal levels of protein (48% crude protein) and lipid (12% crude lipid): a diet based on fishmeal (referred to as Group FM), a diet containing 7% β-conglycinin (referred to as Group 7S), a diet containing 10% glycinin (referred to as Group 11S), a β-conglycinin diet supplemented with 2% alanine-glutamine (referred to as Group CCgGln), and glycinin diet supplemented with 2% alanine-glutamine (referred to as Group GcGln). Healthy hybrid groupers (8.50 ± 0.01 g) were fed each diet for 8 weeks. After the feeding trial finished, distal intestine (DI) samples from the three groups were obtained to determine proteome.

ORGANISM(S): Epinephelus Fuscoguttatus X Epinephelus Lanceolatus

SUBMITTER: Shuyan Chi

PROVIDER: PXD044757 | iProX | Wed Aug 23 00:00:00 BST 2023

REPOSITORIES: iProX

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Publications

An integrated study of glutamine alleviates enteritis induced by glycinin in hybrid groupers using transcriptomics, proteomics and microRNA analyses.

He Yuanfa Y Dong Xiaohui X Yang Qihui Q Liu Hongyu H Zhang Shuang S Xie Shiwei S Chi Shuyan S Tan Beiping B

Frontiers in immunology 20231122

Glutamine has been used to improve intestinal development and immunity in fish. We previously found that dietary glutamine enhances growth and alleviates enteritis in juvenile hybrid groupers (<i>Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂</i>). This study aimed to further reveal the protective role of glutamine on glycinin-induced enteritis by integrating transcriptome, proteome, and microRNA analyses. Three isonitrogenous and isolipidic trial diets were formulated: a diet containing ...[more]

PMID: 38077360

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Project description:Ecdysteroids are a class of steroid hormones occurring in insects, where they are referred as zooecdysteroids, and in plants, where they are termed phytoecdysteroids. While zooecdysteroids in insects regulate important developmental processes, such as embryogenesis, molting (ecdysis), metamorphosis, reproduction, and diapause, phytoecdysteroids provide protection against invertebrate predators by acting as feeding deterrents and by disrupting critical developmental processes of such invertebrates. The ecdysteroids in insects and plants comprise a great number of different analogues, with 20‐hydroxyecdysone, also called ecdysterone, being the quantitatively dominating biologically active analogue in both, insects and plants. Although phytoecdysteroids are effective toxins or antifeedants towards non-adapted herbivorous invertebrate predators, ecdysteroids are apparently non-toxic to mammals and even have been shown to exert a variety of interesting metabolic actions, such as antiobesity, hypoglycemic and protein-anabolic effects. In the present study aimed to test the hypothesis that ecdysterone causes lipid-lowering effects in obese Zucker rats. To test this hypothesis, two groups of obese Zucker rats were fed a nutrient-adequate diet supplemented without or with 0.05% ecdysterone. This ecdysterone concentration was appropriate to achieve a similar dose of ecdysterone per kg body weight BW as applied in other rodent studies, in which ecdysterone caused either hepatic and plasma lipid-lowering effects or antiobesity effects in different rodent models. In order to decipher the potential lipid-lowering actions of ecdysteroids in Zucker rats, measurements of liver and plasma lipid concentrations and hepatic transcriptome analysis was carried out. To further study if ecdysterone is capable of alleviating the strong lipid-synthetic activity in the liver of obese Zucker rats, the study included also two groups of lean Zucker rats which also received either the ecdysterone-supplemented or the non-supplemented diet.

Dataset Information

Quantitative proteomics reveals the protective mechanism of glutamine against enteritis in hybrid groupers

Publications

An integrated study of glutamine alleviates enteritis induced by glycinin in hybrid groupers using transcriptomics, proteomics and microRNA analyses.

Similar Datasets

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