Dataset Information

Proteomic analysis of chronic skin wounds in diabetic mice following electrical stimulation therapy delivered by a novel electrostimulation-hydrogel wound dressing

ABSTRACT: In this study, we established a type 2 diabetes model in C57BL/6 mice through high-fat diet induction and STZ injection. Based on this animal model, we simulated clinically common hard-to-heal chronic diabetic skin wounds by implanting a mixed bacterial fluid, including Methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa (PAO1). We treated these wounds with a novel wearable photovoltaic cell-MEMS electrode-hydrogel dressing (PMH dressing), with commercially available Tegaderm film serving as a control, to explore the therapeutic effects of electrical stimulation on chronic wounds. After 22 days of treatment, we collected wound tissues from each group and conducted proteomic studies using nano LC-MS/MS technology in data independent acquisition (DIA) mode. This project is a repeat study of project PXD040252 after experimental condition optimization, and serves as revision data for the manuscript.

ORGANISM(S): Mus Musculus

SUBMITTER: Yuheng Wang

PROVIDER: PXD044920 | iProX | Tue Aug 29 00:00:00 BST 2023

REPOSITORIES: iProX

ACCESS DATA

Publications

Flexible Organic Photovoltaic-Powered Hydrogel Bioelectronic Dressing With Biomimetic Electrical Stimulation for Healing Infected Diabetic Wounds.

Hu Yi-Wei YW Wang Yu-Heng YH Yang Fang F Liu Ding-Xin DX Lu Guang-Hao GH Li Sheng-Tao ST Wei Zhi-Xiang ZX Shen Xiang X Jiang Zhuang-De ZD Zhao Yi-Fan YF Pang Qian Q Song Bai-Yang BY Shi Ze-Wen ZW Shafique Shareen S Zhou Kun K Chen Xiao-Lian XL Su Wen-Ming WM Jian Jia-Wen JW Tang Ke-Qi KQ Liu Tie-Long TL Zhu Ya-Bin YB

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20231225 10

Electrical stimulation (ES) is proposed as a therapeutic solution for managing chronic wounds. However, its widespread clinical adoption is limited by the requirement of additional extracorporeal devices to power ES-based wound dressings. In this study, a novel sandwich-structured photovoltaic microcurrent hydrogel dressing (PMH dressing) is designed for treating diabetic wounds. This innovative dressing comprises flexible organic photovoltaic (OPV) cells, a flexible micro-electro-mechanical sys ...[more]

PMID: 38145346

Similar Datasets

Project description:Diabetic wounds require continuous and coordinated modulation of the microenvironment concurrent with tissue regeneration, yet this remains a significant challenge. As a proof of concept, we herein propose to use dimeric copper peptide for diabetic wound treatment. The dimeric copper peptide (D-CuP) was first synthesized and then incorporated into a reactive oxygen species (ROS)-responsive hydrogel matrix to improve therapeutic compliance, culminating in the formulation of G/D-CuP. Compared to monomer copper peptide (CuP), a wound healing agent, D-CuP exhibits multivalency, enhanced biologic stability against proteases and the broad biological activities, such as anti-inflammatory and antioxidative properties, while promoting angiogenesis and fibroblast proliferation and migration. Meanwhile, the hydrogel matrix, exhibiting excellent ROS-scavenging capabilities, has been engineered to an intelligent drug reservoir for wound-responsive release of D-CuP at the wound site while simultaneously attenuating inflammatory responses. Ultimately, the G/D-CuP group demonstrated superior therapeutic efficacy, achieving in 97.2% closure of infected wounds.In this study, we present a proof-of-concept study to optimize the treatment of diabetic wounds. This method integrates a dimeric copper peptide hydrogel (G/D-CuP) to address several critical factors: i) The design and synthesis of the dimeric copper peptide (D-CuP) feature a broad range of targets and enhanced biological activities, such as anti-inflammatory and antioxidative properties, while promoting angiogenesis and fibroblast proliferation and migration; ii) Utilizing the steric hindrance conferred by dimerization reduces protease access to the active site and significantly improving stability against proteases; iii) A ROS-responsive hydrogel matrix has been synthesized, exhibiting superior ROS-scavenging capabilities, functioning as an intelligent drug reservoir that enables the controlled release of D-CuP at the wound interface while simultaneously attenuating inflammatory responses in the microenvironment; iv) G/D-CuP possesses outstanding deformability and spreadability, allowing it to adapt to any wound shape; v) The synthesis and preparation process of G/D-CuP are straightforward and cost-effective, making it highly promising for clinical translation. This multifunctional treatment platform is anticipated to accommodate the complex and dynamic biological processes involved in diabetic wound healing, thereby significantly improving the overall efficacy of wound healing treatments.

Dataset Information

Proteomic analysis of chronic skin wounds in diabetic mice following electrical stimulation therapy delivered by a novel electrostimulation-hydrogel wound dressing

Publications

Flexible Organic Photovoltaic-Powered Hydrogel Bioelectronic Dressing With Biomimetic Electrical Stimulation for Healing Infected Diabetic Wounds.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets