Proteomic analysis of chronic skin wounds in diabetic mice following electrical stimulation therapy delivered by a novel electrostimulation-hydrogel wound dressing

ABSTRACT: In this study, we established a type 2 diabetes model in C57BL/6 mice through high-fat diet induction and STZ injection. Based on this animal model, we simulated clinically common hard-to-heal chronic diabetic skin wounds by implanting a mixed bacterial fluid, including Methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa (PAO1). We treated these wounds with a novel wearable photovoltaic cell-MEMS electrode-hydrogel dressing (PMH dressing), with commercially available Tegaderm film serving as a control, to explore the therapeutic effects of electrical stimulation on chronic wounds. After 22 days of treatment, we collected wound tissues from each group and conducted proteomic studies using nano LC-MS/MS technology in data independent acquisition (DIA) mode. This project is a repeat study of project PXD040252 after experimental condition optimization, and serves as revision data for the manuscript.

ORGANISM(S): Mus Musculus

SUBMITTER: Yuheng Wang  

PROVIDER: PXD044920 | iProX | Tue Aug 29 00:00:00 BST 2023

REPOSITORIES: iProX