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Pseudomonas putida as saviour for troubled Synechococcus elongatus in a synthetic co-culture – Interaction studies based on a multi-OMICs approach

ABSTRACT: In their natural environments, microbes rarely exist in isolation; instead, they thrive in consortia where diverse interactions take place. In this study, a defined synthetic co-culture of the cyanobacterium S. elongatus cscB, which feeds the heterotrophic P. putida cscRABY with sucrose, was investigated to identify potential interactions. In initial experiments, a remarkable growth-promoting effect brought about by the presence of the heterotrophic partner on the cyanobacterium was observed, leading to a growth rate increase of up to 80% and increased photosynthetic capacity. Vice versa, the cyanobacterium had a neutral effect on P. putida cscRABY, highlighting the resilience against stress of Pseudomonads and their potential as co-culture partners. A suitable reference process reinforcing the growth-promoting effect was established in a parallel operating photobioreactor system, which sets the basis for the analysis of the co-culture at transcriptional, proteomic, and metabolomic level. This multi-OMICs approach revealed several moderate changes, including alterations in the metabolism, transportation, and stress response in both microbes, indicating multi-level interactions. These findings provide valuable insights into the complex dynamics within the co-culture system, suggesting the exchange of further molecules beyond the unidirectional feeding with sucrose.

ORGANISM(S): Synechococcus Elongatus Pcc 7942 = Fachb-805 Pseudomonas Putida

SUBMITTER: Jagroop Pandhal

PROVIDER: PXD045253 | iProX | Sun Sep 10 00:00:00 BST 2023

REPOSITORIES: iProX

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Pseudomonas putida as saviour for troubled Synechococcus elongatus in a synthetic co-culture - interaction studies based on a multi-OMICs approach.

Kratzl Franziska F Urban Marlene M Pandhal Jagroop J Shi Mengxun M Meng Chen C Kleigrewe Karin K Kremling Andreas A Pflüger-Grau Katharina K

Communications biology 20240412 1

In their natural habitats, microbes rarely exist in isolation; instead, they thrive in consortia, where various interactions occur. In this study, a defined synthetic co-culture of the cyanobacterium S. elongatus cscB, which supplies sucrose to the heterotrophic P. putida cscRABY, is investigated to identify potential interactions. Initial experiments reveal a remarkable growth-promoting effect of the heterotrophic partner on the cyanobacterium, resulting in an up to 80% increase in the growth r ...[more]

PMID: 38609451

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Dataset Information

Pseudomonas putida as saviour for troubled Synechococcus elongatus in a synthetic co-culture – Interaction studies based on a multi-OMICs approach

Publications

Pseudomonas putida as saviour for troubled Synechococcus elongatus in a synthetic co-culture - interaction studies based on a multi-OMICs approach.

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