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Proteomics for ferroptosis-related and CYR61-related molecules

ABSTRACT: This project contains 5 subprojects as to explore role of CYR61 in ferroptosis. In project 1 and 5, lung cancer cells treated with DMSO, RSL3 or erastin for 24 h were collected to perform TMT in order to screen ferroptosis-related molecules. LC-MS/MS in project 2 and 3 were based on immunoprecipitation. anti-CYR61 or anti-ETFA antibodies were used to perform IP with extracted mitochondrial proteins. SAS-PAGE was performed to separate proteins. Gel strips were then cut by panel to perform LC-MS/MS to identify interacted proteins. In project4, blue native-PAGE (BNE) was first applied to separate mitochondrial complexes. Gels strips of target complexes were then isolated to perform LC-MS/MS as to identify components of target complexes.

ORGANISM(S): Homo Sapiens

SUBMITTER: Wanxin Guo

PROVIDER: PXD045540 | iProX | Wed Sep 20 00:00:00 BST 2023

REPOSITORIES: iProX

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