Dataset Information

Sequencing of the mouse testis proteome

ABSTRACT: The present study analyzed the acute inflammatory response of the testis to viral infection using a proteome approach

ORGANISM(S): Mus Musculus

SUBMITTER: Jinlian Hua

PROVIDER: PXD045748 | iProX | Thu Sep 28 00:00:00 BST 2023

REPOSITORIES: iProX

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Publications

A multi-omics analysis of viral nucleic acid poly(I:C) responses to mammalian testicular stimulation.

Yang Donghui D Wu Wenping W Lu Qizhong Q Mou Yaling Y Chen Wenbo W Wan Shicheng S Zhang Mengfei M Wang Congliang C Du Xiaomin X Li Na N Hua Jinlian J

Stress biology 20240201 1

The male reproductive system has a standard immune response regulatory mechanism, However, a variety of external stimuli, including viruses, bacteria, heat, and medications can damage the testicles and cause orchitis and epididymitis. It has been shown that various RNA viruses are more likely to infect the testis than DNA viruses, inducing orchitis and impairing testicular function. It was found that local injection of the viral RNA analog poly(I:C) into the testes markedly disrupted the structu ...[more]

PMID: 38300431

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Project description:Hepatitis A virus (HAV) is a hepatotropic human picornavirus that has been associated only with acute infection. Its pathogenesis is not well understood since there have been few recent studies in animal models using modern methodologies. We characterized HAV infections in three chimpanzees, quantifying viral RNA by qRT-PCR and examining critical aspects of the innate immune response including intrahepatic interferon-stimulated gene expression. We compared these infection profiles with similar studies of chimpanzees infected with hepatitis C virus (HCV), a hepatotropic flavivirus that frequently causes persistent infection. Surprisingly, HAV-infected animals exhibited very limited induction of type I interferon-stimulated genes in the liver compared to chimpanzees with acute resolving HCV infection, despite similar levels of viremia and 100-fold greater quantities of viral RNA in the liver. Minimal ISG15 and IFIT1 responses peaked 1-2 weeks after HAV challenge, then subsided despite continuing high hepatic viral loads. An acute inflammatory response at 3-4 weeks correlated with the appearance of virus-specific antibodies, and both apoptosis and proliferation of hepatocytes. Despite this, HAV RNA persisted in the liver for months, remaining present long after its clearance from serum and feces and revealing dramatic differences in the kinetics of clearance in the three compartments. Viral RNA was detected in the liver for significantly longer (35 to >48 weeks) than HCV RNA in animals with acute resolving HCV infection (10-20 weeks). Collectively, these findings suggest that early HAV infection is far stealthier than HCV infection and represents a distinctly different paradigm in viral-host interactions within the liver. Chimpanzee liver was biopsied during an acute HAV infection. Chimp 1 and 2 had two baseline samples. Chimp 3 used the baselines from chimps 1 and 2. Chimp 1 had 8 samples during the HAV acute infection. Chimp 2 had 9 samples during the HAV acute infection. Chimp 3 had 4 samples during the HAV acute infection.

Dataset Information

Sequencing of the mouse testis proteome

Publications

A multi-omics analysis of viral nucleic acid poly(I:C) responses to mammalian testicular stimulation.

Similar Datasets

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