Project description:Determine the comprehensive miRNA expression profile in RNA extracted from whole blood samples from healthy controls (CTRL), individuals positive to CCP antibodies >25UI/ml (CCP+), early rheumatoid arthritis (ERA) and established rheumatoid arthritis (CRA) according to the ACR/EULAR 2010 classification criteria. Several miRNAs were overexpressed on ERA patients. We selected 5 miRNAs (miR-361-5p, miR-23a-3p, miR-223-3p, miR-4634 and miR-128-3p) for its quantification on a bigger group of subjects by RT-qPCR and the results showed good concordance with microarray expression data.
Project description:This SuperSeries is composed of the following subset Series: GSE32016: Autoantibody Epitope Spreading in the Pre-Clinical Phase Predicts Progression to Rheumatoid Arthritis [ANALYTE: ANTIGEN] GSE32019: Autoantibody Epitope Spreading in the Pre-Clinical Phase Predicts Progression to Rheumatoid Arthritis [ANALYTE: Cytokine or chemokine] Refer to individual Series
Project description:We found the bone marrow stromal-derived neural progenitor cells secretome have the neural protection effect. Proteomic analysis was performed nn order to analyze the protection factor in the secretome. Keywords: Neural protection, secretome
Project description:Rheumatoid factor are autoantibodies that are found in +/- two thirds of patients with rheumatoid arthritis, a chronic autoimmune disease characterized by inflamed, swollen joints. They consist of polyclonal antibodies that target the Fc part of IgG. The antibodies are not highly specific for rheumatoid arthritis and clinical assays to measure rheumatoid factor are poorly harmonized. We studied rheumatoid factor using a mass-spectrometry-based approach in seropositive, seronegative rheumatoid arthritis patients and in disease controls. Rheumatoid factor was captured on Fc coated microwell plates, isolated, digested into peptides and analyzed by liquid chromatography tandem mass spectrometry. Principal component analysis and sparse partial least squares discriminant analysis showed that peptides derived from seropositive rheumatoid arthritis patients clustered away from the controls. Mass spectrometry analysis revealed framework region-derived and variable region-derived peptides that were enriched in rheumatoid factor positive sera. However, mass spectrometry de novo sequencing failed to sequence the majority of unidentified peptides. Furthermore, mass spectrometry analysis revealed different rheumatoid factor isotypes. In addition to IgM, also IgA and IgG isotypes were observed. In conclusion, mass spectrometry is able to capture the complexity and isotypes of rheumatoid factor autoantibodies, but advances in de novo sequencing are needed to fully characterize the variable part of the antibodies.
Project description:It has been suggested that CD4+ T cell senescence is involved in the pathogenesis of elderly-onset rheumatoid arthritis (EORA). We performed scRNA-seq analysis using CD4+ T cells collected from inguinal lymph nodes of young and old DBA/1 mice to investigate the association between aging CD4+ T cells and elderly-onset arthritis.
Project description:Tumor Necrosis Factor Alpha is a known pro-inflammatory cytokine that plays a key role in the pathogenesis of rheumatoid arthritis. Anti-cytokine therapies targeting Tumor Necrosis Factor Alpha have greatly succeeded in treating rheumatoid arthritis in many patients. Despite these developments, many of the mechanisms of Tumor Necrosis Factor Alpha action have yet to be uncovered. In this study, we incubated CD3+ T-cells from healthy donors and rheumatoid arthritis patients with Tumor Necrosis Factor Alpha and then performed their single-cell multi-omics analysis via BD Rhapsody.