Proteomics

Dataset Information

0

FGL1 IP-MS data in 293T cell lysates

ABSTRACT: We immunoprecipitated exogenously expressed Myc-FGL1 using Anti-Myc-Tag monoclonal antibody (DIA-AN, Wuhan, China, Cat#2097) from 293T cells cocultured with tumor associated macrophages (TAMs) for 16 hr. No FGL1 protein was identified in protein lysates in which normal mouse IgG (Santa Cruz, California, USA, Cat#sc-2025) was added. Thus, protein lysates of 293T cells cocultured with TAMs added to mouse IgG were utilized as the negative control. A high-throughput technology, LC/MS (MS), was applied to identify the candidate interacting proteins of FGL1.

ORGANISM(S): Homo Sapiens

SUBMITTER: Huaiqiang Ju  

PROVIDER: PXD045948 | iProX | Tue Oct 03 00:00:00 BST 2023

REPOSITORIES: iProX

Json Xml
altmetric image

Publications

The liver microenvironment orchestrates FGL1-mediated immune escape and progression of metastatic colorectal cancer.

Li Jia-Jun JJ   Wang Jin-Hong JH   Tian Tian T   Liu Jia J   Zheng Yong-Qiang YQ   Mo Hai-Yu HY   Sheng Hui H   Chen Yan-Xing YX   Wu Qi-Nian QN   Han Yi Y   Liao Kun K   Pan Yi-Qian YQ   Zeng Zhao-Lei ZL   Liu Ze-Xian ZX   Yang Wei W   Xu Rui-Hua RH   Ju Huai-Qiang HQ  

Nature communications 20231023 1

Colorectal cancer (CRC) patients with liver metastases usually obtain less benefit from immunotherapy, and the underlying mechanisms remain understudied. Here, we identify that fibrinogen-like protein 1 (FGL1), secreted from cancer cells and hepatocytes, facilitates the progression of CRC in an intraportal injection model by reducing the infiltration of T cells. Mechanistically, tumor-associated macrophages (TAMs) activate NF-ĸB by secreting TNFα/IL-1β in the liver microenvironment and transcrip  ...[more]

Similar Datasets

Proteomics CCDC188 IP-MS data in mouse testis protein lysates
2023-09-28 | PXD045762 |
Genomics C57Bl6J retina lysates
| PRJNA870946 | ENA
Proteomics Kindlin-2 A549 Nano-LC-MS/MS Analysis
2018-12-30 | MSV000083291 | MassIVE
Transcriptomics Effect of embryo lysates treatment on gene expression.
2024-09-07 | GSE265886 | GEO
Genomics Effect of embryo lysates treatment on small RNA expression
2024-09-07 | GSE265889 | GEO
Genomics C57Bl6J retina lysates in oxygen-induced retinopathy
| PRJNA666571 | ENA
Genomics Analysis of RNA-TAG labeling in HeLa lysates
| PRJNA627877 | ENA
Genomics Effect of embryo lysates treatment on gene expression.
| PRJNA1104627 | ENA
Genomics RNA sequencing from hippocampal total lysates of the rTg4510 tauopathy mouse model.
| PRJNA772726 | ENA
Transcriptomics Analysis of RNA-TAG labeling in HeLa lysates
2020-04-25 | GSE149274 | GEO