FGL1 IP-MS data in 293T cell lysates

ABSTRACT: We immunoprecipitated exogenously expressed Myc-FGL1 using Anti-Myc-Tag monoclonal antibody (DIA-AN, Wuhan, China, Cat#2097) from 293T cells cocultured with tumor associated macrophages (TAMs) for 16 hr. No FGL1 protein was identified in protein lysates in which normal mouse IgG (Santa Cruz, California, USA, Cat#sc-2025) was added. Thus, protein lysates of 293T cells cocultured with TAMs added to mouse IgG were utilized as the negative control. A high-throughput technology, LC/MS (MS), was applied to identify the candidate interacting proteins of FGL1.

ORGANISM(S): Homo Sapiens

SUBMITTER: Huaiqiang Ju  

PROVIDER: PXD045948 | iProX | Tue Oct 03 00:00:00 GMT+01:00 2023

REPOSITORIES: iProX