Proteomics

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FGL1 IP-MS data in 293T cell lysates

ABSTRACT: We immunoprecipitated exogenously expressed Myc-FGL1 using Anti-Myc-Tag monoclonal antibody (DIA-AN, Wuhan, China, Cat#2097) from 293T cells cocultured with tumor associated macrophages (TAMs) for 16 hr. No FGL1 protein was identified in protein lysates in which normal mouse IgG (Santa Cruz, California, USA, Cat#sc-2025) was added. Thus, protein lysates of 293T cells cocultured with TAMs added to mouse IgG were utilized as the negative control. A high-throughput technology, LC/MS (MS), was applied to identify the candidate interacting proteins of FGL1.

ORGANISM(S): Homo Sapiens

SUBMITTER: Huaiqiang Ju  

PROVIDER: PXD045948 | iProX | Tue Oct 03 00:00:00 BST 2023

REPOSITORIES: iProX

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Publications

The liver microenvironment orchestrates FGL1-mediated immune escape and progression of metastatic colorectal cancer.

Li Jia-Jun JJ   Wang Jin-Hong JH   Tian Tian T   Liu Jia J   Zheng Yong-Qiang YQ   Mo Hai-Yu HY   Sheng Hui H   Chen Yan-Xing YX   Wu Qi-Nian QN   Han Yi Y   Liao Kun K   Pan Yi-Qian YQ   Zeng Zhao-Lei ZL   Liu Ze-Xian ZX   Yang Wei W   Xu Rui-Hua RH   Ju Huai-Qiang HQ  

Nature communications 20231023 1

Colorectal cancer (CRC) patients with liver metastases usually obtain less benefit from immunotherapy, and the underlying mechanisms remain understudied. Here, we identify that fibrinogen-like protein 1 (FGL1), secreted from cancer cells and hepatocytes, facilitates the progression of CRC in an intraportal injection model by reducing the infiltration of T cells. Mechanistically, tumor-associated macrophages (TAMs) activate NF-ĸB by secreting TNFα/IL-1β in the liver microenvironment and transcrip  ...[more]

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