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Interaction of species A rotavirus VP4 with the cellular proteins vimentin and actin related protein 2 discovered by a proximity interactome assay

ABSTRACT: Rotavirus (RV) is one of the most significant pathogens in humans and animals with diarrhea worldwide. Cell entry is the first step in viral infection, and the outer capsid protein VP4 is crucial for RV attachment and internalization. In order to discover novel candidate host factors involved in RV cell entry, a proximity labeling method was applied to systematically investigate the VP4 and host protein interactions. A total of 174 high confidence host proteins were identified using proximity labeling. Further analysis showed that 88 proteins were located in the cytoskeleton, plasma membrane, and extracellular region, which could be involved in RV entry.

ORGANISM(S): Homo Sapiens

SUBMITTER: Chang Li

PROVIDER: PXD045952 | iProX | Sat Oct 07 00:00:00 BST 2023

REPOSITORIES: iProX

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Interaction of species A rotavirus VP4 with the cellular proteins vimentin and actin related protein 2 discovered by a proximity interactome assay.

Hao Pengfei P Qu Qiaoqiao Q Pang Zhaoxia Z Li Letian L Du Shouwen S Shang Limin L Jin Chaozhi C Xu Wang W Ha Zhuo Z Jiang Yuhang Y Chen Jing J Gao Zihan Z Jin Ningyi N Wang Jian J Li Chang C

Journal of virology 20231122 12

<h4>Importance</h4>Rotavirus (RV) is an important zoonosis virus, which can cause severe diarrhea and extra-intestinal infection. To date, some proteins or carbohydrates have been shown to participate in the attachment or internalization of RV, including HGBAs, Hsc70, and integrins. This study attempted to indicate whether there were other proteins that would participate in the entry of RV; thus, the RV VP4-interacting proteins were identified by proximity labeling. After analysis and verificati ...[more]

PMID: 37991368

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