Dataset Information

A quantitative proteomic study reveals oxidative stress and synaptic loss contributed to TDCIPP exposure induced memory impairment (2)

ABSTRACT: Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) has been consistently identified in various environmental media and biological specimens. The current understanding of the in vivo toxicities of TDCIPP remains limited, especially for its neurotoxicity and cognitive impairment effects. To better evaluate the potential damage of TDCIPP on learning and memory, Sprague Dawley (SD) Rats were subjected to TDCIPP exposure at doses of 40, 120, and 360 mg/kg/day for a duration of 90 days. Quantitative proteomic analysis, immunohistochemistry, and western blotting techniques were employed to assess alterations in proteins following exposure to TDCIPP. Open field test and Morris Water Mase tests were used to assess anxiety and spatial learning memory capacity in rats. In the present study, it was observed that the administration of TDCIPP induced anxiety and cognitive impairments in rats. Additionally, a noteworthy decrease in the number of neurons was observed in the hippocampal CA3 and DG regions. Subsequent bioinformati

ORGANISM(S): Rattus Rattus

SUBMITTER: Benhong Xu

PROVIDER: PXD045959 | iProX | Sun Oct 08 00:00:00 BST 2023

REPOSITORIES: iProX

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A quantitative proteomic study reveals oxidative stress and synapse-related proteins contributed to TDCIPP exposure induced neurotoxicity.

Zou Chunli C Yang Tingting T Zhang Jiuhong J Chen Xiao X Zhao Jing J Wu Desheng D Yang Chen C Liu Peiyi P Huang Xinfeng X Liu Jianjun J Xu Benhong B

Ecotoxicology and environmental safety 20240122

Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) has been consistently identified in various environmental media and biological specimens. Current understanding of the in vivo toxicities of TDCIPP is limited, especially for potential for neurotoxic and cognitive impairment effects. To better evaluate the potential adverse effect of the chemical on learning and memory, Sprague Dawley (SD) rats were administered TDCIPP via gavage at doses of 40, 120, and 360 mg/kg/day for a period of 90 days. Quanti ...[more]

PMID: 38262093

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Project description:Aging is often associated with cognitive decline, but many elderly individuals maintain a high level of function throughout life. Here we studied outbred rats, which also exhibit individual differences across a spectrum of outcomes that includes both preserved and impaired spatial memory. Previous work in this model identified the CA3 subfield of the hippocampus as a region critically affected by age and integral to differing cognitive outcomes. Earlier microarray profiling revealed distinct gene expression profiles in the CA3 region, under basal conditions, for aged rats with intact memory and those with impairment. Because prominent age-related deficits within the CA3 occur during neural encoding of new information, here we used microarray analysis to gain a broad perspective of the aged CA3 transcriptome under activated conditions. Behaviorally induced CA3 expression profiles differentiated aged rats with intact memory from those with impaired memory. In the activated profile, we observed substantial numbers of genes (greater than 1000) exhibiting increased expression in aged unimpaired rats relative to aged impaired, including many involved in synaptic plasticity and memory mechanisms. This unimpaired aged profile also overlapped significantly with a learning induced gene profile previously acquired in young adults. Alongside the increased transcripts common to both young learning and aged rats with preserved memory, many transcripts behaviorally-activated in the current study had previously been identified as repressed in the aged unimpaired phenotype in basal expression. A further distinct feature of the activated profile of aged rats with intact memory is the increased expression of an ensemble of genes involved in inhibitory synapse function, which could control the phenotype of neural hyperexcitability found in the CA3 region of aged impaired rats. These data support the conclusion that aged subjects with preserved memory recruit adaptive mechanisms to retain tight control over excitability under both basal and activated conditions. RNA profiles from cognitively unimpaired and impaired aged rats were compared under 2 conditions: spatial learning task and a non-spatial learning task.

Dataset Information

A quantitative proteomic study reveals oxidative stress and synaptic loss contributed to TDCIPP exposure induced memory impairment (2)

Publications

A quantitative proteomic study reveals oxidative stress and synapse-related proteins contributed to TDCIPP exposure induced neurotoxicity.

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