Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Uterine tissue protein composition analysis during the estrous interphase

ABSTRACT: Uterine tissue was obtained from healthy SD rats during the estrous interphase and subjected to protein composition analysis using LC‒MS.

ORGANISM(S): Rattus Norvegicus

SUBMITTER: Li Liu

PROVIDER: PXD046009 | iProX | Tue Oct 10 00:00:00 BST 2023

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Similar Datasets

Uterine glands impact uterine receptivity, luminal fluid homeostasis and blastocyst implantation

Project description:Uterine glands are essential for pregnancy in mice and likely humans, because they secrete or transport bioactive substances that regulate uterine receptivity for blastocyst implantation. In mice, the uterus becomes receptive to blastocyst implantation on day 4, but is refractory by day 5. Here, blastocysts could be recovered from progesterone-induced uterine gland (PUGKO) but not wildtype (WT) mice on day 5 post-mating. Anti-adhesive Muc1 protein and microvilli were present on the luminal epithelium of PUGKO but not WT uteri. A number of known uterine receptivity genes and gland-specific genes were altered in the PUGKO uterus. Next, the uterus and uterine luminal fluid (ULF) were obtained from WT and PUGKO mice on day 3, 4 and 5. Transcriptome analysis revealed that 580 genes were decreased in the PUGKO uterus, however ULF secrotome analysis revealed that many proteins and several amino acids were increased in the PUGKO ULF. Of note, many proteins encoded by many gland-specific genes were not identified in the ULF of WT mice. These results support the ideas that uterine glands secrete factors that regulate ULF homeostasis and interact with other cell types in the uterus to influence uterine receptivity and blastocyst implantation for the establishment of pregnancy.

2016-11-22 | GSE90101 | GEO

Bovine uterine fluid extracellular vesicle proteome on different timepoints of the oestrous cycle

Project description:Healthy cow uterine fluid (UF) was collected from synchronized cattle at day 0, 7 and 16 after ovulation. Extracellular vesicles (EVs) were isolated from UF. UF-EVs from same cattle on different timepoints were subjected to LC-MS/MS. Additionally, one sample before EV purification was subjected to LC-MS/MS (corresponding EV sample is named 1D0).

2024-01-26 | PXD040201 | Pride

Effect of neonatal right ventricular pressure overload on gene expression and regions of open chromatin during postnatal heart development

Project description:To investigate the effect of pressure overload (PO) during postnatal right ventricular (RV) development, we established the RV PO model by conducting pulmonary artery banding (PAB) surgery on neonatal Sprague-Dawley (SD) rats. We then performed gene expression and chromatin openness profiling analysis using data obtained from RNA-seq and ATAC-seq of RV free walls from PO and sham-operated rats at postnatal day 21 (P21).

2024-05-17 | GSE210180 | GEO

Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty

Project description:Identification of puberty-associated cell composition and characterization of the unique transcriptional signatures across different cells are beneficial to specific neurons isolation and advanced understanding their functions. In this study, the whole brains of female SD rats aged PND-25, 35 and 45were performed 10 μm serial tissue sections transversely to expose ARC regions (bregma: -2.52 to 2.92 mm, interaural: 6.08 to 6.48 mm) according to Allen Brain Atlas and processed by spatial transcriptomics sequencing.

2021-11-07 | E-MTAB-10976 | biostudies-arrayexpress

Uterine microbiota

Project description:Uterine microbiota Metagenome

| PRJNA480713 | ENA

rat gut metagenome

Project description:Gut microbiota composition of rats subjected to desynchronosis

| PRJNA760288 | ENA

Transcription profiling of human uterine fibroid (leiomyoma) and normal myometrial tissue to identify differentially expressed genes

Project description:Our study seeks to identify genes differentially expressed between uterine leiomyomas and normal myometrial tissue. This series consists of samples derived from normal myometrium and uterine leiomyomas obtained from fibroid afflicted patients.Total RNA was extracted from samples, converted to biotin-labeled cRNA, hybridized to oligonucleotide arrays, and followed by model based expression analysis.,In order to select differentially expressed genes of interest, dChip model-based expression analysis was employed. Significant genes were identified, utilizing the dChip software, as having an average fold change of > +1.5 or < -1.5 between leiomyoma and normal myometrium and p < 0.05. Under these conditions the 226 genes in the following list were identified.

2007-09-28 | E-GEOD-593 | biostudies-arrayexpress

Genome-Wide Progesterone Receptor Binding: Cell Type-Specific and Shared Mechanisms in Uterine Fibroids and Breast Cancer

Project description:Analysis of genes regulated by RU486 (an progesterone antagonist) in human breast cancer T47D cells and human uterine leiomyoma smooth muscle cells. The hypothesis is that RU486 inhibits tumor growth by inactivating the transcription of multiple genes which trigger critical signaling pathways to induce tumorigenesis in both breast caner and uterine leomyoma. Tissue-specific and common patterns of gene regulation may determine the therapeutic effects of antiprogestins in uterine leiomyoma and breast cancer. Total RNA isolated from T47D cells subjected to RU486 treatment for 6 hours compared to vehicle (ethanol) treated cells. Total RNA isolated from uterine leiomyoma cells subjected to RU486 treatment for 6 hours compared to vehicle (ethanol) treated cells.

2011-07-22 | E-GEOD-30871 | biostudies-arrayexpress

Uterine extracellular vesicles in the mare.

Project description:The protein cargos of uterine extracellular vesicles isolated from uterine lavages, collected from pregnant mares (P; day 10, 11, 12 and 13 after insemination) and cyclic control mares (C; day 10 and 13 after insemination), was analyzed.

2022-10-15 | PXD032405 | Pride

Age and Nursing Affect the Neonatal Porcine Uterine Transcriptome

Project description:Age- and lactocrine-sensitive elements of the neonatal porcine uterine developmental program are undefined. Here, effects of age and nursing for 48 h from birth (postnatal day = [PND] 0) on the uterine transcriptome at PND 2 were identified using RNA sequencing. Pig uterine tissues were obtained from neonatal gilts (n = 4/group) within 1 h of birth [postnatal day (PND) 0], or 48 h after nursing ad libitum (PND 2N), or feeding a commercial milk replacer (PND 2R).

2016-02-15 | E-GEOD-72388 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data