Proteomics

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Chromatin Isolation by RNA Purification (ChIRP) assays followed by LC-MS in DCRT-KO cell lines


ABSTRACT: To explore the molecular mechanism of DCRT in DCM, Chromatin Isolation by RNA Purification (ChIRP) assays followed by Liquid Chromatography Mass Spectrometry (LC-MS) analyses were conducted. Each probe was designed using the web-based designer provided by Sangon Biotech, biotin-TEG-labeled separated into odd and even groups. Probes that targeted LacZ were utilized as a safeguard. For each sample, 4 × 10^7 WT or DCRT-KO AC16 cells were used and crosslinked in 1% glutaraldehyde at room temperature for 10 min, and quenched with 125 mM glycine for 5 min. Cleared lysates were hybridized with probes in 37 °C in a hybridization oven for 4 h. After washes and elution, proteins were purified and LC-MS

ORGANISM(S): Homo Sapiens

SUBMITTER: Chen Chen  

PROVIDER: PXD046020 | iProX | Tue Oct 10 00:00:00 BST 2023

REPOSITORIES: iProX

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