Screening for the transcriptional factors interacted with Id1 in RAW264.7 cells

ABSTRACT: Proteins in RAW264.7 cells were extracted by CO-IP lysis buffer, Id1 antibody and agarose gel beads were added into the lysis buffer and rotated in 4°C overnight. The agarose gel beads were washed for 5 times by centrifugation, and denatured in 2 ✖️loading buffer. The denatured protein samples were sent for MS analysis and screened for the transcriptional factors interacted with Id1.

ORGANISM(S): Mus Musculus

SUBMITTER: Fang Hua  

PROVIDER: PXD046173 | iProX | Tue Oct 17 00:00:00 BST 2023

REPOSITORIES: iProX