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[HPP-2.0]A simple, rapid, and practical method for single-cell proteomics based on mass-adaptive coating of synthetic peptides

ABSTRACT: Here, we report a simple, rapid, and practical approach of mass-adaptive coating-assisted single-cell proteomics (Mad-CASP). Combined with the designed synthetic peptides and novel data acquisition strategy, 1240 ± 93 (n = 3) proteins were identified from a single HeLa cell by Mad-CASP.

ORGANISM(S): Homo Sapiens

SUBMITTER: Jin Zi

PROVIDER: PXD046177 | iProX | Tue Oct 17 00:00:00 BST 2023

REPOSITORIES: iProX

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A simple, rapid, and practical method for single-cell proteomics based on mass-adaptive coating of synthetic peptides.

Li Siqi S Su Kaichan K Zhuang Zhenkun Z Qin Qing Q Gao Lei L Deng Yamei Y Liu Xuyang X Hou Guixue G Wang Longtao L Hao Piliang P Yang Huanming H Liu Siqi S Zhu Hongming H Ren Yan Y

Science bulletin 20211228 6

PMID: 36546118

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