Ejection of mitochondria and TNF-α activation mediate hypertonicity-induced adipocytes dedifferentiation

ABSTRACT: Adipocyte extracellular vesicles and particles (EVs) proteomic profiling. In this study, we focus on the EVs proteomes changes induced by hypertonicity in 3T3-L1 and differentiated adipocytes. The source cells were cultured with isotonic/hypertonic medium for 48 hours, and EVs were isolated from the conditional medium using sequential ultracentrifugation. A label-free MS strategy was employed to achieve proteome identification and quantification.

ORGANISM(S): Mus Musculus

SUBMITTER: Liang Zhang  

PROVIDER: PXD046259 | iProX | Fri Oct 20 00:00:00 GMT+01:00 2023

REPOSITORIES: iProX