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The antibacterial mechanism of Loratadine derivative lo-7 against Streptococcus agalactiae

ABSTRACT: Streptococcus agalactiae isolate was inoculated and cultured to exponential growth phase (OD600 at 0.5) in TSB and divided into two groups, the control group and the drug treated group. Loratadine derivative lo-7 was added to the drug treated group until a final concentration of 1/2× MIC reached, and DMSO added in the control group. Then, cultures in both groups were cultivated for 2 h at 200 rpm. The microbial was centrifuged at 12000 rpm for 10 min, washed twice with Phosphate Buffer Saline (PBS). Lyse the cells by bead-beating at 4 °C in RIPA lysis buffer (1% Triton X-100, 1% deoxycholate, 0.1% SDS), The protein reduction, alkylation and tryptic digestion were followed as filter-aided sample preparation (FASP). The digestion products were analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), followed by label-free quantitative analysis. The MS experiment was performed on a Q Exactive plus mass spectrometer.

ORGANISM(S): Streptococcus Agalactiae Atcc 13813

SUBMITTER: Zewen Wen

PROVIDER: PXD046298 | iProX | Fri Oct 20 00:00:00 BST 2023

REPOSITORIES: iProX

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