Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A Deep Proteome Analysis Identifies the Antimicrobial Activity of Royal Jelly Against Escheria coli

ABSTRACT: Royal jelly (RJ) from honeybees is known to be composed of diverse antibacterial components with broad-spectrum antibacterial activity. In this study, a model organism of bacteria (Escherichia coli) was challenged with RJ and the proteomic changes were investigated. A total of 209 differentially expressed proteins (DEPs) were identified; 200 (95.70%) DEPs were upregulated and 9 (4.30%) were downregulated. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were applied.

ORGANISM(S): 大肠杆菌

SUBMITTER: Yue Hao

PROVIDER: PXD046406 | iProX | Thu Oct 26 00:00:00 BST 2023

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Similar Datasets

MicroRNA profiling of royal jelly extracellular vesicles and their potential role in cell viability and reversing cell apoptosis

Project description:We provide a detailed analysis of miRNA in the royal jelly nanovesicles.

2023-12-28 | GSE219219 | GEO

Proteomic Analysis of Xinjiang Black Bee's Royal Jelly Using the Osboren Classification Method

Project description:Royal jelly has long been recognized as health food, with a high content of proteins. These proteins play important roles in honeybee caste and human health, but the proteomic analysis of those low-abundance proteins in royal jelly is always a challenge. Herein, we used the Osboren classification method to separate the royal jelly proteins of Xinjiang black bees, a sub-species of Apis mellifera mellifera, into various fractions. The globulin, ethanol-soluble protein and glutelin fractions were further separated by SDS-PAGE, and proteomic analysis was carried out by LC-MS/MS and searching against the NCBI database. A total of 63 proteins with definitive names were identified, in which 41 proteins were identified for the first time in royal jelly. The Osboren classification method combined with one-dimensional gel electrophoresis based proteomic analysis allows the identification of low-abundance proteins, and greatly extends the knowledge about the components and functions of royal jelly proteins.

2024-08-09 | PXD023315 | Pride

Quantitative comparison of manuka and clover honey proteomes with royal jelly

Project description:SWATH-MS was used to compare relative protein quantities of bee origin in manuka and clover honey to royal jelly.

2023-02-12 | PXD038889 | Pride

RNA-Seq of full-length RNA from worker and royal jelly of honey bees (Apis mellifera)

Project description:Characterization of naturally-occurring RNAs (up to 200nt) in the honey bee worker and royal jelly

2018-11-29 | E-MTAB-6557 | biostudies-arrayexpress

Metagenome of the microbiota of royal jelly

Project description:Metagenome of the microbiota of royal jelly

| PRJNA358558 | ENA

honeybee biology and Royal jelly functionality

Project description:Functional assessment of Royal jelly on honeybee larvae development and

2021-03-30 | PXD025090 |

Transcriptomics of Royal Jelly Composition in Apidae

Project description:Transcriptomics of Royal Jelly Composition in Geotrigona acapulconis and apis mellifera

| PRJEB60465 | ENA

Gene expression analysis of the hippocampus in ovariectomized mice following royal jelly administration

Project description:Postmenopausal women may experience not only physical symptoms such as hot flashes, night sweats, and bone loss, but also psychiatric symptoms such as anxiety and depression. Hormone replacement therapy is used to treat menopausal symptoms; however, no significant beneficial effects on psychiatric symptoms have been established. In recent years, naturally derived compounds have attracted attention as effective and safe therapeutic options. One such compound, royal jelly (RJ), has anti-inflammatory, antioxidant, neuroprotective, and estrogen-like properties, and has been suggested to improve anxiety- and depression-related behaviors in postmenopausal models; however, the mechanisms underlying these effects are unclear. In this study, to clarify the efficacy of RJ against menopause-associated psychiatric symptoms and its underlying mechanisms, we analyzed changes in gene expression in the hippocampus, a brain region associated with emotional regulation, using an ovariectomized (OVX) mouse model.

2026-05-09 | GSE327513 | GEO

RNA-seq of total royal jelly and MRJP-3-bound RNA

Project description:MRJP-3 is an RNA binding protein that binds both double- and single-stranded RNA in vitro. To test whether i) MRJP-3 binds RNA in its natural environment, the royal jelly (RJ) and ii) to characterize its RJ RNA partners; a pull down followed by RNA-seq system was developed. MRJP-3 bound RNA was compared to total RJ RNA extracted from the same hive. To establish the system, we first incubated biotinylated MRJP-3, or biotinylated BSA in RJ and pulled the proteins out with strepavidin coated magnetic beads followed by RNA extraction and bioanalyzer profiling. MRJP-3 pull out was enriched for RNA compare to the BSA and just beads controls. The RNA population pulled down with MRJP-3 has similar profile to the total RJ RNA, further demonstrating the MRJP-3 has no affinity to a certain RNA species.

2018-10-28 | E-MTAB-6732 | biostudies-arrayexpress

Mouse VSMC LC-MSMS - Functional and Proteomic Investigations Reveal Major Royal Jelly Protein 1 Associated with Anti-hypertension Activity in Mouse Vascular Smooth Muscle Cells

Project description:Vascular smooth muscle cells (VSMCs) are a major cell type of the arterial wall and their functionality is associated with blood pressure regulation. Although royal jelly (RJ) has reported effects on anti-hypertension, the mechanism of blood pressure regulation by major royal jelly protein 1 (MRJP1), the most abundant RJ protein, is still unknown. Therefore, mrjp1 gene was delivered into mouse VSMCs to investigate how MRJP1 influences the VSMC functionality by functional and proteomic analysis.The data here are the proteomic analysis of triplicated control and MRJP1 expressing VSMCs.

2018-10-27 | PXD004207 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data