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A Comprehensive Proteomics Analysis Reveals Dynamic Phenotypes of Tumor-associated Macrophages and Their Precursor Cells in Tumor Progression

ABSTRACT: Tumor-associated macrophages (TAMs) are key regulators in tumor progression. Although a role of bone marrow-derived monocytes (Mons) as TAM precursors has been revealed, the dynamic phenotypes of both TAMs and Mons regulated by the tumor microenvironment remain unclear. Here, we constructed an optimized micro-proteomics workflow, which was applicable to the mouse myeloid cells of low cell number. We sorted both TAMs and the corresponding Mons (1×105 per sample) from individual melanoma mouse model in the early stage and the late stage to establish the protein expression profiles by mass spectrum.

ORGANISM(S): Mus Musculus

SUBMITTER: Jian Wang

PROVIDER: PXD046492 | iProX | Mon Oct 30 00:00:00 GMT 2023

REPOSITORIES: iProX

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Comprehensive Proteomics Analysis Reveals Dynamic Phenotypes of Tumor-Associated Macrophages and Their Precursor Cells in Tumor Progression.

Liu Ying Y Liu Di D Liu Yuchen Y Fu Bin B Ji Shuhui S Wang Ruixuan R Yan Fang F Wang Huan H Zhao Dianyuan D Yang Wenting W Wang Jian J Tang Li L

Journal of proteome research 20240103 2

Tumor-associated macrophages (TAMs) are key regulators in tumor progression, but the precise role of bone marrow-derived monocytes (Mons) as TAM precursors and their dynamic phenotypes regulated by the tumor microenvironment (TME) remain unclear. Here, we developed an optimized microproteomics workflow to analyze low-cell-number mouse myeloid cells. We sorted TAMs and their corresponding Mons (1 × 10<sup>5</sup> per sample) from individual melanoma mouse models at both the early and late stages. ...[more]

PMID: 38173118

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