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A Comprehensive Proteomics Analysis Reveals Dynamic Phenotypes of Tumor-associated Macrophages and Their Precursor Cells in Tumor Progression

ABSTRACT: Tumor-associated macrophages (TAMs) are key regulators in tumor progression. Although a role of bone marrow-derived monocytes (Mons) as TAM precursors has been revealed, the dynamic phenotypes of both TAMs and Mons regulated by the tumor microenvironment remain unclear. Here, we constructed an optimized micro-proteomics workflow, which was applicable to the mouse myeloid cells of low cell number. We sorted both TAMs and the corresponding Mons (1×105 per sample) from individual melanoma mouse model in the early stage and the late stage to establish the protein expression profiles by mass spectrum.

ORGANISM(S): Mus Musculus

SUBMITTER: Jian Wang

PROVIDER: PXD046492 | iProX | Mon Oct 30 00:00:00 GMT 2023

REPOSITORIES: iProX

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