Proteomics

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Mapping start codons of small open reading frames by N-terminomics approach


ABSTRACT: we used N-terminomics approach to identify the N-terminal sequences of SEPs in Hep3B cells. Three SEPs enrichment methods (HCl precipitation, heating precipitation and ACN precipitation), three chemical derivatization methods (dimethylation, acetylation and guanidylation) and three N-terminal peptides enrichment materials (hexadecaldehyde, CNBr activated agarose and NHS activated agarose) were tested in this experiment. We also determined the start codons of SEPs with their N-terminal information.

ORGANISM(S): Homo Sapiens

SUBMITTER: Cuihong Wan  

PROVIDER: PXD046680 | iProX | Mon Nov 06 00:00:00 GMT 2023

REPOSITORIES: iProX

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