Mapping start codons of small open reading frames by N-terminomics approach
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ABSTRACT: we used N-terminomics approach to identify the N-terminal sequences of SEPs in Hep3B cells. Three SEPs enrichment methods (HCl precipitation, heating precipitation and ACN precipitation), three chemical derivatization methods (dimethylation, acetylation and guanidylation) and three N-terminal peptides enrichment materials (hexadecaldehyde, CNBr activated agarose and NHS activated agarose) were tested in this experiment. We also determined the start codons of SEPs with their N-terminal information.
ORGANISM(S): Homo Sapiens
SUBMITTER:
Cuihong Wan
PROVIDER: PXD046680 | iProX | Mon Nov 06 00:00:00 GMT 2023
REPOSITORIES: iProX
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