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Proteomics reveals the significance of vacuole Pi transporter in the adaptability of Brassica napus to Pi deprivation

ABSTRACT: A proteomic analysis was performed to estimate the role of BnPHT5;1a in Pi fluctuation. Results showed that Pi deprivation disturbed the physiological processes involved in carbohydrate metabolism, response to stimulus and stress in B. napus, while disruption of BnPHT5;1a genes exacerbated these processes. Besides, the processes of cell redox homeostasis, lipid metabolic and proton transmembrane transport were also unbalanced in BnPHT5;1a DM under the -Pi condition. Noteworthy, disruption of BnPHT5;1a genes severely altered ATP biosynthesis, and proton/inorganic cation transmembrane under normal Pi condition, which might contribute to B. napus growth limitations. Additionally, seven new protein markers of Pi homeostasis were identified in B. napus.

ORGANISM(S): Brassica Napus

SUBMITTER: Lei Shi

PROVIDER: PXD047074 | iProX | Thu Nov 16 00:00:00 GMT 2023

REPOSITORIES: iProX

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Proteomics reveals the significance of vacuole Pi transporter in the adaptability of Brassica napus to Pi deprivation.

Han Bei B Yan Junjun J Wu Tao T Yang Xinyu X Wang Yajie Y Ding Guangda G Hammond John J Wang Chuang C Xu Fangsen F Wang Sheliang S Shi Lei L

Frontiers in plant science 20240325

Vacuolar Pi transporters (VPTs) have recently been identified as important regulators of cellular Pi status in Arabidopsis thaliana and Oryza sativa. In the oil crop Brassica napus, BnA09PHT5;1a and BnC09PHT5;1a are two homologs of AtPHT5;1, the vacuolar Pi influx transporter in Arabidopsis. Here, we show that Pi deficiency induces the transcription of both homologs of PHT5;1a genes in B. napus leaves. Brassica PHT5;1a double ...[more]

PMID: 38590751

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