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Generation of a deep mouse brain spectral library for transmembrane proteome profiling in mental disease models

ABSTRACT: The establishment of a high-quality mouse brain transmembrane proteins spectral library enables in-depth profiling of the proteins across multiple brain regions. DIA data analysis, combining different search pipeline based on the spectral library, discovered a new GPCR regulator which was associated with depression verified via in vivo tests. This library represents a valuable resource that has the potential to discover the putative drug targets.

ORGANISM(S): Mus Musculus

SUBMITTER: Wenqing Shui

PROVIDER: PXD047251 | iProX | Sun Nov 26 00:00:00 GMT 2023

REPOSITORIES: iProX

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Generation of a Deep Mouse Brain Spectral Library for Transmembrane Proteome Profiling in Mental Disease Models.

Li Shanshan S Luo Huoqing H Tang Pan P Tian Cuiping C Hu Ji J Lu Haojie H Shui Wenqing W

Molecular & cellular proteomics : MCP 20240425 6

Transmembrane (TM) proteins constitute over 30% of the mammalian proteome and play essential roles in mediating cell-cell communication, synaptic transmission, and plasticity in the central nervous system. Many of these proteins, especially the G protein-coupled receptors (GPCRs), are validated or candidate drug targets for therapeutic development for mental diseases, yet their expression profiles are underrepresented in most global proteomic studies. Herein, we establish a brain TM protein-enri ...[more]

PMID: 38670310

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