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Comparative proteomics of fresh sperm and frozen sperm collected by electric stimulation method and Artificial vagina method

ABSTRACT: This progect included TMT-based proteomics and PRM proteomics. The study aimed to we aimed to investigate the differential impact of rectal probe electrostimulation and artificial vagina semen collection methods on the quality and the freezing tolerance of goat sperm.

ORGANISM(S): Capra Hircus

SUBMITTER: Chunyan Li

PROVIDER: PXD047354 | iProX | Wed Nov 29 00:00:00 GMT 2023

REPOSITORIES: iProX

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Integrating proteomics and metabolomics to evaluate impact of semen collection techniques on the quality and cryotolerance of goat semen.

Li Chunyan C Liang Jiachong J Allai Larbi L Badaoui Bouabid B Shao Qingyong Q Ouyang Yina Y Wu Guoquan G Quan Guobo G Lv Chunrong C

Scientific reports 20241127 1

Results of artificial insemination (AI) are affected by changes in sperm quality and the function throughout collection and preservation procedures. Proteome and metabolome alterations of sperm treated with the different procedures in goat, however, aren't fully understood. To this end, we sought to investigate the impacts of rectal probe electrostimulation (EE) and artificial vagina (AV) semen collection methods on the quality and the cryotolerance of goat sperm, with additional focus on proteo ...[more]

PMID: 39604559

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