Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

EIF3H-interactome in Colorectal Cancer cell line (HCT116)

ABSTRACT: To elucidate the molecular mechanism and search for EIF3H substrate(s) of EIF3H-mediated CRC progression, we performed immune-affinity purification and resolved the binding partners utilizing mass spectrometry. HCT116 cells transfected with empty vector or Flag-tagged EIF3H and then immunoprecipitated using anti-Flag M2 beads.The purified EIF3H protein complex was subjected to mass spectrometry analysis.

ORGANISM(S): Homo Sapiens

SUBMITTER: Monghong Lee

PROVIDER: PXD048151 | iProX | Tue Dec 26 00:00:00 GMT 2023

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Similar Datasets

HAX1-interactome in Colorectal Cancer cell line (HCT116)

Project description:We performed immune-affinity purification and resolved the binding partners utilizing mass spectrometry to elucidate the molecular mechanism of HAX1-mediated CRC progression. HCT116 cells transfected with empty vector or Flag-HAX1 and then immunoprecipitated using anti-Flag M2 beads. The purified HAX1 protein complex was subjected to mass spectrometry analysis.

2024-01-11 | PXD048485 |

HCT116 cells N-BLR shRNA vs. empty vector control

Project description:HCT116 cells transfected with lentiviral vectors expressing two different N-BLR shRNAs (clones #3-1 and #4-7) and empty vector control

2016-12-27 | GSE92915 | GEO

Transcription profiling of human colon cancer cell line HCT116 expressing miR-43a vs. controls to investigate miR-43a induced gene expression

Project description:In order to examine the consequences of human miR-34a induction on the transcriptome, HCT116 cells (a colon cancer cell line) were infected with a retrovirus that produces miR-34a. Gene expression profiles were then monitored using Affymetrix microarrays. Affymetrix microarrays were used to examine the transcriptomes of HCT116 cells infected with an empty retroviral vector (pMSCV-PIG) or a retroviral vector that expresses human miR-34a. Experiment Overall Design: RNA from retrovirally-infected cells was isolated and hybridized to Affymetrix microarrays.

2008-06-15 | E-GEOD-7754 | biostudies-arrayexpress

mRNA-seq analysis of HCT116 cells following gene overexpression of IDO1 with or without Indole treatment

Project description:To investigate the effect of indole on protection against colon injury and the role of IDO1 expression, we examined the effect of indole on hIDO1-overexpressing and empty vector control HCT116 cells by global gene expression profiling.

2016-07-01 | GSE83939 | GEO

RIT1 oncoproteins escape LZTR1-mediated proteolysis

Project description:These data accompany our publication "RIT1 oncoproteins escape LZTR1-mediated proteolysis" (Castel P., et al. Science, 2019). Experiment 1: Identification of LZTR1 as an interacting partner of RIT1 Q Exactive Plus P20161208-02 FLAG GFP P20161208-03 FLAG RIT1 LTQ Orbitrap Velos V20170407-01 GST empty vector V20170407-03 GST RIT1 V20170407-06 HA empty vector V20170407-07 HA RIT1 Experiment 2: Identification of K135 and K187 ubiquitination sites in RIT1 Q Exactive Plus P20180408-01 FLAG-RIT1 / empty vector / empty vector (replicate 1) P20180408-02 FLAG-RIT1 / LZTR1 / empty vector (replicate 1) P20180408-03 FLAG-RIT1 / empty vector / TUBE (replicate 1) P20180408-04 FLAG-RIT1 / LZTR1 / TUBE (replicate 1) P20180408-06 GST-RIT1 / empty vector / empty vector (replicate 1) P20180408-07 GST-RIT1 / LZTR1 / empty vector (replicate 1) P20180408-08 GST-RIT1 / empty vector / TUBE (replicate 1) P20180408-09 GST-RIT1 / LZTR1 / TUBE (replicate 1) P20180408-11 GST-RIT1 / empty vector / empty vector (replicate 2) P20180408-12 GST-RIT1 / LZTR1 / empty vector (replicate 2) P20180408-13 GST-RIT1 / empty vector / TUBE (replicate 2) P20180408-14 GST-RIT1 / LZTR1 / TUBE (replicate 2) P20180408-16 FLAG-RIT1 / empty vector / empty vector (replicate 2) P20180408-17 FLAG-RIT1 / LZTR1 / empty vector (replicate 2) P20180408-18 FLAG-RIT1 / empty vector / TUBE (replicate 2) P20180408-32 FLAG-RIT1 / LZTR1 / TUBE (replicate 2)

2019-02-14 | MSV000083444 | MassIVE

Shotgun analysis of proteins co-immunoprecipitating with OCIAD1-FLAG

Project description:Interactome of OCIAD1-FLAG was studied. TMT-based relative quantification of protein levels was performed in eluates from mitochondria of HEK293 cells that expressed OCIAD1-FLAG vs empty vector and from mitochondria of OCIAD1-KO cells that expressed OCIAD1-FLAG vs empty vector.

2024-11-08 | PXD043863 | Pride

Overexpression of CYGB led to differential gene expression in HCT116 cells (RNA-seq)

Project description:The goal of this study was to define the cohort of genes whose expression is regulated by CYGB function in colorectal cancer. Towards this goal, HCT116 cells stably expressing CYGB (HCT-CYGB) were established. In addition, a control cells (HCT-MOCK) was generated by transfection with empty vector. Subsequently, total RNA was isolated and then performed transcriptome RNA sequence.

2021-04-12 | GSE149426 | GEO

RNF5 interactome

Project description:Cells expressing Flag-tagged RNF5 or empty vector were incubated with the proteasome inhibitor MG132 to block protein degradation. Cell lysates were then prepared and immunoprecipitationed with anti-Flag antibody were performed, and eluates were digested with trypsin and analyzed on a Q Exactive.

2018-11-25 | MSV000083160 | MassIVE

Outer membrane proteins mediate the Pseudomonas unconventional peroxidase secretion by crosstalk between the Sec pathway and outer membrane vesicles

Project description:To elucidate the non canonical secretion mechanism of DypB2985, co immunoprecipitation coupled with mass spectrometry was first employed to identify potential interacting partners of DypB2985. The assay was conducted using Pseudomonas putida A514 harboring a FLAG tagged DypB2985 vector, with A514 carrying the empty FLAG tagged vector serving as the control.

2025-10-31 | MSV000099705 | MassIVE

RNA-seq for HCT116 cells with TTC22-OE or kockdown

Project description:object:compare the genes influenced by TTC22 overexpression or knockdown Methods: For whole-genome transcriptome profiling, four libraries were generated from total RNA samples extracted from Flag-TTC22-OE, empty vector control, scramble RNA control, and TTC22-KD (siTTC#1&2&3) HCT116 cells using TruSeq® RNA Sample Preparation Kit (Illumina Inc.) according to the manufacturer’s protocol, and sequenced on the Illumina HiSeq PE150 platform (Genminix, Shanghai, China) using the 150–base pair single-end sequencing module. Hisat2 (version:2.0.4) was used to map the cleaned reads to the human hg38 reference genome. Results: The mRNA data obtained from transcriptome sequencing, subjected to T-test statistics and corrected by the RVM model. Significant differential mRNA (TwoClassDif) between TTC22-OE and vector control cells or between TTC22-KD and scramble RNA control cells was yielded. Up-regulated and down-regulated genes (fold change>1.5, calculated through Ballgown algorithm) were performed to find significant functions and significant signaling pathways based on the Gene Ontology database (GO-Analysis) or KEGG database.(Pathway-Analysis). Conclusion:The results suggested that TTC22 could affect the RNA metabolism pathway that controls the progression and metastasis of CC.

2024-12-01 | GSE189892 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data