Quantitative phosphoproteomics explain cryopreservation-induced reductions in ram sperm motility
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ABSTRACT:
ORGANISM(S): Ovis Aries
SUBMITTER: 
Yi Fang
PROVIDER: PXD048166 | iProX | Thu Dec 28 00:00:00 GMT 2023
REPOSITORIES: iProX
Publications
Quantitative phosphoproteomics explain cryopreservation-induced reductions in ram sperm motility.
Zang Shengqin S Yang Xiaorui X Ye Jiangfeng J Mo Xianhong X Zhou Guangbin G Fang Yi Y
Journal of proteomics 20240302
Sperm cryopreservation decreases motility, probably due to changes in protein phosphorylation. Our objective was to use quantitative phosphoproteomics for systematic comparative analyses of fresh versus frozen-thawed sperm to identify factors causing cryo-injury. Ejaculates were collected (artificial vagina) from six Dorper rams, pooled, extended, and frozen over liquid nitrogen. Overall, 915, 3382, and 6875 phosphorylated proteins, phosphorylated peptides, and phosphorylation sites, respectivel ...[more]
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