Project description:To investigate the effects of OMVs and IN@OMVs treatments on mouse tumor-associated macrophages, mouse tumors were harvested after treatment, and CD11b+ F4/80+ labeled cells (TAMs) were sorted by FACS for RNA sequencing.
Project description:Characterization of the sRNA content of P. aeruginosa OMVs compared to whole cells. Result: OMVs contain differentially packaged sRNAs. Whole cell PA14 and OMVs from 3 separate preparations.
Project description:In this study, we purified and characterized two types of H.pylori OMVs (standard strain NCTC11637 and clinical strain Hp-400) and analyzed the proteome using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).
Project description:Characterization of the sRNA content of P. aeruginosa OMVs compared to whole cells. Result: OMVs contain differentially packaged sRNAs.
Project description:5′ tRNA-fMet1 half (5′- CGCGGGGTGGAGCAGTCTGGTAGCTCGTCGGGCTC-3′) was cloned into the arabinose-inducible expression vector pMQ70. PA14 was transformed with the tRNA-fMet1 half expression vector or empty vector via electroporation. Characterization of the sRNA content of OMVs secreted by 5' tRNA-fMet1 half over-expression clone and empty vector control clone.
Project description:The alveolar epithelial cell injury is essential for the initaitation and maintenance of inflammatory of lung in ARDS. Outer membrane vesicles (OMVs) are nanoparticles produced by the extrusion of the outer membrane of gram-negative bacteria deliver a range of bacterial molecules to host cells. Recent findings describe OMVs play vital roles in bacterial colonization, delivery of virulence factors, and disease pathogenesis. We here report OMVs as a key regulator of gene expression in primary alveolar epithelial cell.
Project description:To better examine the molecular mechanisms behind the virus infection, we conducted a correlation analysis of RNA-Seq and quantitative iTRAQ-LC-MS/MS in TuMV-infected and in healthy Chinese cabbage leaves.