Dataset Information

Porphyromonas gingivalis infection and atherosclerosis

ABSTRACT: To explore the effect of Porphyromonas gingivalis on lipid-laden macrophages, the project was conduct. Overall design: 8 samples, 4 replicates in 2 groups, with ox-LDL-treated RAW264.7 macrophages stimulated with or without Porphyromonas gingivalis.

ORGANISM(S): Mus Musculus

SUBMITTER: Lili Chen

PROVIDER: PXD048371 | iProX | Tue Jan 09 00:00:00 GMT 2024

REPOSITORIES: iProX

ACCESS DATA

Publications

Porphyromonas gingivalis aggravates atherosclerotic plaque instability by promoting lipid-laden macrophage necroptosis.

Huang Xiaofei X Xie Mengru M Wang Yixuan Y Lu Xiaofeng X Mei Feng F Zhang Kaiwen K Yang Xinlong X Chen Guangjin G Yin Ying Y Feng Guangxia G Song Wencheng W Dong Nianguo N Deng Xuliang X Wang Songling S Chen Lili L

Signal transduction and targeted therapy 20250523 1

At advanced phases of atherosclerosis, the rupture and thrombogenesis of vulnerable plaques emerge as primary triggers for acute cardiovascular events and fatalities. Pathogenic infection such as periodontitis-associated Porphyromonas gingivalis (Pg) has been suspected of increasing the risks of atherosclerotic cardiovascular disease, but its relationship with atherosclerotic plaque destabilization remains elusive. Here we demonstrated that the level of Pg-positive clusters positively correlated ...[more]

PMID: 40404630

Similar Datasets

Project description:Porphyromonas gingivalis is a major pathogen associated with the microbial biofilm-mediated disease chronic periodontitis. P. gingivalis has an obligate requirement for iron and protoporphyrin IX which it satisfies by transporting heme and iron liberated from the human host. The level of cellular iron in P. gingivalis affects the expression of a distinct iron-associated regulon of 64 genes and low iron invokes an iron sparing response. Iron homeostasis is usually mediated in Gram-negative bacteria at the transcriptional level by the Ferric Uptake Regulator (Fur). There is a single predicted P. gingivalis Fur superfamily orthologue named Har (heme associated regulator) that lacks the conserved metal binding residues found in other Fur orthologues. We show that Har binds both heme and ferrous iron resulting in a conformational change in the protein. Har was unable to complement the Escherichia coli H1780 fur mutant and there was no change in cellular metal content in a P. gingivalis Har mutant compared with the wild-type. The Har regulon of 44 genes is not predicted to play a role in iron homeostasis. Together these data indicated that Har does not regulate iron homeostasis in P. gingivalis. However, Har was required for heme-responsive biofilm development and its regulon overlapped P. gingivalis regulons previously identified after growth in heme limitation or as a homotypic biofilm. P. gingivalis is unique as an iron-dependent Gram-negative bacterium with a single heme-binding Fur superfamily orthologue, Har, that does not regulate iron homeostasis. Paired samples were compared on the same microarray using a two-colour system. A total of 6 paired microarray hybridizations were performed representing 6 biological replicates, where a balanced dye design was used, with the overall analysis including three microarrays where P. gingivalis 33277 samples were labeled with Cy3 and the paired ECR455 samples were labeled with Cy5 and three other microarrays where samples were labeled with the opposite combination of fluorophores.

Dataset Information

Porphyromonas gingivalis infection and atherosclerosis

Publications

Porphyromonas gingivalis aggravates atherosclerotic plaque instability by promoting lipid-laden macrophage necroptosis.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets