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Integrated Single-Cell Proteomics Analysis on an Active Matrix Digital Microfluidic Chip

ABSTRACT: We introduce and implement a comprehensive single-cell proteomics sample pre-treatment solution based on an active matrix digital microfluidics chip(AM-DMF-SCP). This platform accommodates high-throughput single-cell isolation and seamless, non-destructive sample pre-treatment, characterized by its efficiency, speed, stability, and ease of use, thereby overcoming the longstanding bottleneck in single-cell protein sample preparation.

ORGANISM(S): Homo Sapiens

SUBMITTER: Hu Zhou

PROVIDER: PXD048434 | iProX | Mon Apr 15 00:00:00 BST 2024

REPOSITORIES: iProX

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AM-DMF-SCP: Integrated Single-Cell Proteomics Analysis on an Active Matrix Digital Microfluidic Chip.

Yang Zhicheng Z Jin Kai K Chen Yimin Y Liu Qian Q Chen Hongxu H Hu Siyi S Wang Yuqiu Y Pan Zilu Z Feng Fang F Shi Mude M Xie Hua H Ma Hanbin H Zhou Hu H

JACS Au 20240326 5

Single-cell proteomics offers unparalleled insights into cellular diversity and molecular mechanisms, enabling a deeper understanding of complex biological processes at the individual cell level. Here, we develop an integrated sample processing on an active-matrix digital microfluidic chip for single-cell proteomics (AM-DMF-SCP). Employing the AM-DMF-SCP approach and data-independent acquisition (DIA), we identify an average of 2258 protein groups in single HeLa cells within 15 min of the liquid ...[more]

PMID: 38818059

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