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Binding protein of IGFBP2 3'UTR with or without m6A methylation

ABSTRACT: RNA pull-down assay using 4 pairs of m6A methylated or unmethylated single-stranded RNAs carrying part of the 3′-UTR sequence of IGFBP2 (~20 bp in length) as baits, followed by mass spectrometry analysis. Each pair of RNA baits contained one of the 4 putative m6A consensus motifs in the 3′-UTR of IGFBP2

ORGANISM(S): Homo Sapiens

SUBMITTER: Yonghui Li

PROVIDER: PXD048441 | iProX | Thu Jan 11 00:00:00 GMT 2024

REPOSITORIES: iProX

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m6A in 5â untranslated regions promotes cap-independent translation of mRNA

Project description:N6-methyladenosine (m6A) is a widespread internal RNA modification whose function is poorly understood. Here we report that m6A residues within the 5'UTR promote a novel form of cap-independent translation which is mediated through an interaction between m6A residues and the translation initiation factor, eIF3. We present eIF3a PAR-iCLIP data which demonstrate that eIF3 predominantly binds mRNAs within the 5'UTR. eIF3 binding sites are also in proximity to m6A residues within the 5'UTR of cellular mRNAs. Two replicates of eIF3a PAR-iCLIP in HEK293T cells.

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Widespread Occurrence of m6A in bacterial mRNA

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m6A RNA methylation promotes XIST-mediated transcriptional repression

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