Dataset Information

Comparison of serum proteomics reveals mechanism underlying immune response after transplantation in Pinctada fucata

ABSTRACT: By using the "borrowing belly to nurture pearls" technology, cultivating Pinctada maxima pearls on Pinctada fucata can increase the yield of high−quality pearls. However, this heterologous transplantation is prone to causing immune−related mortality. To overcome this technical flaw, researchers are striving to explore the potential mechanisms of immune responses after allograft and xenograft in pearl oysters through proteomic. Through LC−MS/MS analysis, more than 2000 proteins were successfully identified in the serum of Pinctada fucata after transplantation surgery.

ORGANISM(S): Pinctada Fucata

SUBMITTER: Pei Wang

PROVIDER: PXD048483 | iProX | Fri Jan 12 00:00:00 GMT 2024

REPOSITORIES: iProX

ACCESS DATA

Similar Datasets

Project description:Immune responses induced by ongoing and/or past infections prevent the establishment of transplantation tolerance in experimental animal models. How host-pathogen interactions influence allograft tolerance in humans has not been investigated before. The spontaneous development of operational tolerance in liver transplant recipients with chronic hepatitis C virus (HCV) infection constitutes a unique setting to address this question. We conducted a clinical trial of immunosuppression withdrawal in stable HCV-infected liver recipients to elucidate: i) the mechanisms through which allograft tolerance is established in the presence of an ongoing inflammatory response; and ii) how is influenced by anti-HCV heterologous immune responses. Enrolled patients gradually discontinued their immunosuppressive drugs over 6-9 months, and those who maintained normal allograft status 12 months after drug withdrawal were considered operationally tolerant. Successful drug withdrawal was associated with intra-hepatic over-expression of type I interferon and immune-regulatory genes, and correlated with an expansion of exhausted PD1/CTLA4/2B4-positive HCV-specific circulating CD8+ T cells. These findings were already present before immunosuppression was discontinued and were specific for HCV infection. In contrast, the magnitude of HCV-induced inflammatory gene expression and the scope of anti-HCV effector T cell responses did not influence drug withdrawal outcome. Our data indicate that in humans persistent viral infections do not necessarily preclude the development of transplantation tolerance. At least in HCV-infected liver allografts, mechanisms associated with the capacity of the virus to evade adaptive immunity could contribute to the restraining of alloimmune responses and the establishment of transplantation tolerance. Transcriptomic study from the following liver tissue samples: 12 tolerant before immunosuppression (IS), 13 non-tolerant before IS, 4 from non-tolerant at the time of rejection, 13 from tolerant patients 12 months after IS discontinuation. Additionally, 8 liver tissue samples obtained from healthy living liver donors undergoing partial hepatectomy were included as non-transplanted controls.

Dataset Information

Comparison of serum proteomics reveals mechanism underlying immune response after transplantation in Pinctada fucata

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets