Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparison of differentially expressed proteins and phosphorylated proteins between wild type and Tnni3k splicing mutation lines of zebrafish

ABSTRACT: By eatablished Tnni3k splicing mutation to explore the mechanism of Tnni3k in maintaining cardiac physiological function

ORGANISM(S): Danio Rerio

SUBMITTER: Yun Deng

PROVIDER: PXD048620 | iProX | Tue Jan 16 00:00:00 GMT 2024

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Similar Datasets

Identification of differentially expressed proteins and phosphorylated proteins in rice seedlings in response to strigolactone treatment.

Project description: Not available

| S-EPMC3974870 | biostudies-literature

Proteomic identification of differentially expressed and phosphorylated proteins in epidermis involved in larval-pupal metamorphosis of Helicoverpa armigera.

Project description: Not available

| S-EPMC2806347 | biostudies-literature

Comparison of splicing tools

Project description:Comparison of splicing tools

| PRJNA675664 | ENA

Sex-lethal regulates back-splicing and generation of the sex-differentially expressed circular RNAs

Project description:Conversely to canonical splicing, back-splicing covalently ligates the upstream 3' splice site (SS) with downstream 5'SS and generates exonic circular RNAs (circRNAs) that are widely-identified in eukaryotes and have regulatory functions in gene expression. However, sex-specific back-splicing in Drosophila has not been investigated and its regulation remains unclear. Here, we performed multiple RNA-seq of various sex-specific Drosophila samples including head, body and gonads from both genders, and identified more than ten thousand of circular RNAs, in which hundreds are sex-differentially expressed and back-spliced. Intriguingly, we found that expression of SXL, an RNA-binding protein encoded by Sex-lethal (Sxl), the master Drosophila sex-determination gene which only functionally spliced in females, promotes back-splicing of many female-differentially expressed circRNAs in the male S2 cells, while expression of a SXL mutant did not. Using a monoclonal antibody, we further obtained the transcriptome-wide RNA-binding sites of SXL through a PAR-CLIP approach and revealed that SXL-binding on flanking exons and introns of pre-mRNAs facilitates back-splicing of those circRNAs, whereas SXL-binding on the circRNA exons inhibits the back-splicing. This study provides strong evidence that SXL has a regulatory role in back-splicing to generate sex-specifc circRNAs, as well as in the initiation of Drosophila sex-determination cascade through canoncial forward-splicing.

2023-04-23 | GSE221102 | GEO

Glycoprofile Comparison of the SARS-CoV-2 Spike Proteins Expressed in CHO and HEK Cell Lines.

Project description: Not available

| S-EPMC12413344 | biostudies-literature

Identification of differentially expressed genes in the zebrafish hypothalamic-pituitary axis.

Project description: Not available

| S-EPMC2804439 | biostudies-literature

Chemometrics of differentially expressed proteins from colorectal cancer patients.

Project description: Not available

| S-EPMC3084394 | biostudies-literature

Differentially Expressed Tear Proteins in Sjogren's Syndrome Keratoconjunctivitis Sicca.

Project description: Not available