Proteomics

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Determination of major proteins in the insoluble and soluble fractions of Dermatophagoides farinae using LC-MS/MS


ABSTRACT: The insoluble and soluble fractions of Dermatophagoides farinae proteins were extracted using 8 M urea solution (8 M urea, 4% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, 40 mM Tris base) and 0.1 M PBS solutions, respectively. The main bands in the soluble and insoluble fractions were separately excised from SDS-PAGE gels and were determination using LC-MS/MS.

ORGANISM(S): Dermatophagoides Farinae

SUBMITTER: YaE Zhao  

PROVIDER: PXD048635 | iProX | Wed Jan 17 00:00:00 GMT 2024

REPOSITORIES: iProX

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