Determination of major proteins in the insoluble and soluble fractions of Dermatophagoides farinae using LC-MS/MS
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ABSTRACT: The insoluble and soluble fractions of Dermatophagoides farinae proteins were extracted using 8 M urea solution (8 M urea, 4% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, 40 mM Tris base) and 0.1 M PBS solutions, respectively. The main bands in the soluble and insoluble fractions were separately excised from SDS-PAGE gels and were determination using LC-MS/MS.
ORGANISM(S): Dermatophagoides Farinae
SUBMITTER:
YaE Zhao
PROVIDER: PXD048635 | iProX | Wed Jan 17 00:00:00 GMT 2024
REPOSITORIES: iProX
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