Proteomics

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HIRA and dPCIF1 coordinately establish totipotent chromatin and control orderly ZGA in Drosophila embryos

ABSTRACT: In order to understand the molecular mechanism underlying the action of dPCIF1 in early embryos, we carried out co-immunoprecipitation (co-IP) assays followed by mass spectroscopic analysis using dPCIF1GFP-KI early embryos (0.5–1.5 hours). In this assay, we identified a large number of dPCIF1-interacting proteins. To search for the potential factor(s) with GAF, we performed co-IP assays of early embryos, followed by mass spectroscopic analysis, and generated a dataset for GAF-interacting proteins. To identify the site(s) of dPCIF1 phosphorylation, we performed phosphor-proteomics by mass spectroscopy and identified potential phosphorylation sites.

ORGANISM(S): Drosophila Melanogaster

SUBMITTER: Dahua Chen  

PROVIDER: PXD048659 | iProX | Thu Jan 18 00:00:00 GMT 2024

REPOSITORIES: iProX

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HIRA and dPCIF1 coordinately establish totipotent chromatin and control orderly ZGA in <i>Drosophila</i> embryos.

Zhang Guoqiang G   Miao Yaqi Y   Song Yuan Y   Wang Liangliang L   Li Yawei Y   Zhu Yuanxiang Y   Zhang Wenxin W   Sun Qinmiao Q   Chen Dahua D  

Proceedings of the National Academy of Sciences of the United States of America 20241114 47

Early embryos undergo profound changes in their genomic architecture to establish the totipotent state, enabling pioneer factors to access chromatin and drive zygotic genome activation (ZGA). However, the mechanisms by which the totipotent state is established and properly interpreted by pioneer factors to allow orderly ZGA remain unknown. Here, we identify the H3.3-specific chaperone HIRA as a factor involving establishing totipotent-state chromatin in <i>Drosophila</i> early embryos. Through c  ...[more]

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